Property: Has notes
From Miniscope
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Cortical preparation required tissue aspiration to seat the lens. Cohorts: 6 striatal (3 D1-Cre, 3 A2A-Cre), 4 cortical (2 Syn, 2 CaMKII). +
Implant depth differs by sex (-6.6 mm females, -6.7 mm males) — one of the few entries where the protocol adjusts coordinates for sex. Published as a Current Protocols article, so it is unusually explicit about failure modes. +
Field of view 3.6 x 2.3 mm. Hemisphere was balanced across animals by design rather than fixed. +
Frame rate, gain and LED power were tuned per mouse and then held constant across days, so no single frame rate is reported. A passive commutator (ONE Core, University of Colorado) kept the animal untethered in yaw. A parietal skull screw anchored the headcap. +
Scope cited only as "a Miniscope" (Cai et al. 2016) acquired with the Miniscope DAQ system; no version stated. Tissue above the mPFC was aspirated through a 1 mm radius craniotomy before lens implantation; lens sealed with Kwik-Sil and dental acrylic, and baseplate covered with black dental cement plus a screw-on protective cap. A TTL from the Miniscope DAQ triggered the FLIR behaviour camera (30 fps) through a 6-pin GPIO Hirose cable for frame-by-frame alignment. Delta F/F used 70% neuropil subtraction and a dynamic baseline. Imaging was done primarily in female pairs because post-surgical males could not be reliably re-paired; sex of the imaged animals is not broken down. GCaMP6f and jGCaMP8m animals were pooled in analyses. +
An intersectional strategy: the recorded population is defined by where it projects, not by a genetic marker. +
10 animals implanted, 9 analyzed. The second colour is a static structural label, not a second activity sensor — its purpose is to make cross-session cell identity verifiable rather than inferred from activity. +
A unilateral 26-gauge guide cannula was implanted at a 30 degree angle (AP +1.6, ML -2.65, 2.30 mm DV) in the same surgery so vehicle (ACSF) or IEM-1460 (1 mM, 500 nL) could be infused to the imaged neurons; unilateral infusion was chosen because it left partner preference behaviour intact. No tissue aspiration; a blunt 30-gauge needle tract to -3.6 mm DV preceded slow lens lowering to -4.4 mm over 10 min. Lens protected with Body Double silicone until baseplating. Open Ephys V4 Miniscopes and baseplates, overhead MiniCAM, Miniscope DAQ 3.3 over coaxial cable, Miniscope-DAQ-QT Software v1.11, gain 3.5; cables looped well above the chamber to minimise experimenter intervention and untangling; no commutator mentioned. Animals habituated to the scope for 15 min on three days. Videos downsampled to 15 Hz before analysis. Three animals were lost to scope PCB failures and two to lens/cannula mistargeting. Females were estrogen-primed before mate introduction. Lens placement mapped onto mouse atlas sections; field of view spans medial NAc core and shell. Neuropixels 2.0 recordings (females only) and slice patch-clamp in the same paper are not miniscope experiments. +
ChrimsonR stimulation of medial septum delivered through a separate 200 um fiber (5 ms pulses, scrambled or 8 Hz paced) while imaging CA1 — a worked example of combining a miniscope with an independent optogenetic implant. +
Field of view 3.6 x 2.7 mm. Head-fixed rather than freely behaving, and a cranial window rather than a lens — the same scope covering a very different preparation from the two rat entries on this paper. +
Wire-free DAQ weighs 3.5 g and gives roughly 1 hour of battery life; data written to microSD. Field of view 1.4 x 1.4 mm. Single demonstration animal. +
Field of view ~2.54 mm2. Same rat CA1 preparation later used in Blair et al. 2023. +
Long habituation — at least 3 weeks post-baseplate — before recording. +
A notably short 3-day injection-to-implant interval, at the fast end of anything in this registry. Combined with chemogenetic (DREADD) manipulation. +
Included deliberately as a boundary case: no craniotomy, no lens, no chronic implant. It shows the registry's scope is optical access to a neural population, not the brain specifically. +
Miniscope carried an integrated electrophysiology headstage (E-Scope) for simultaneous cerebellar recording. +
Both animals of a pair were imaged at once, which is the unusual feature of this preparation -- two scopes running simultaneously in a shared arena. Imaging was performed after the pair had learned the task. Behaviour recorded at 15 fps on a FLIR infrared camera and annotated with the Hong lab Behavior Annotator. +
Three separate surgeries: viral injection, lens implant, then baseplate. +
Two different lens geometries were used across the cohort (Inscopix 1 x 9 mm in three animals, Grintech 1.8 x 4.2 mm in two). +
Window placed contralateral to the dominant forelimb. Cohorts: 10 C57BL/6, 7 Cck-/-, 7 wild-type with a CCKBR antagonist. One of the few registry entries using a cranial window rather than a GRIN lens, which is possible here only because the target is superficial. +