Property: Has notes
From Miniscope
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E
Cortical preparation required tissue aspiration to seat the lens. Cohorts: 6 striatal (3 D1-Cre, 3 A2A-Cre), 4 cortical (2 Syn, 2 CaMKII). +
Implant depth differs by sex (-6.6 mm females, -6.7 mm males) — one of the few entries where the protocol adjusts coordinates for sex. Published as a Current Protocols article, so it is unusually explicit about failure modes. +
Field of view 3.6 x 2.3 mm. Hemisphere was balanced across animals by design rather than fixed. +
Frame rate, gain and LED power were tuned per mouse and then held constant across days, so no single frame rate is reported. A passive commutator (ONE Core, University of Colorado) kept the animal untethered in yaw. A parietal skull screw anchored the headcap. +
An intersectional strategy: the recorded population is defined by where it projects, not by a genetic marker. +
10 animals implanted, 9 analyzed. The second colour is a static structural label, not a second activity sensor — its purpose is to make cross-session cell identity verifiable rather than inferred from activity. +
ChrimsonR stimulation of medial septum delivered through a separate 200 um fiber (5 ms pulses, scrambled or 8 Hz paced) while imaging CA1 — a worked example of combining a miniscope with an independent optogenetic implant. +
Field of view 3.6 x 2.7 mm. Head-fixed rather than freely behaving, and a cranial window rather than a lens — the same scope covering a very different preparation from the two rat entries on this paper. +
Wire-free DAQ weighs 3.5 g and gives roughly 1 hour of battery life; data written to microSD. Field of view 1.4 x 1.4 mm. Single demonstration animal. +
Field of view ~2.54 mm2. Same rat CA1 preparation later used in Blair et al. 2023. +
Long habituation — at least 3 weeks post-baseplate — before recording. +
A notably short 3-day injection-to-implant interval, at the fast end of anything in this registry. Combined with chemogenetic (DREADD) manipulation. +
Included deliberately as a boundary case: no craniotomy, no lens, no chronic implant. It shows the registry's scope is optical access to a neural population, not the brain specifically. +
Miniscope carried an integrated electrophysiology headstage (E-Scope) for simultaneous cerebellar recording. +
Both animals of a pair were imaged at once, which is the unusual feature of this preparation -- two scopes running simultaneously in a shared arena. Imaging was performed after the pair had learned the task. Behaviour recorded at 15 fps on a FLIR infrared camera and annotated with the Hong lab Behavior Annotator. +
Three separate surgeries: viral injection, lens implant, then baseplate. +
Two different lens geometries were used across the cohort (Inscopix 1 x 9 mm in three animals, Grintech 1.8 x 4.2 mm in two). +
Window placed contralateral to the dominant forelimb. Cohorts: 10 C57BL/6, 7 Cck-/-, 7 wild-type with a CCKBR antagonist. One of the few registry entries using a cranial window rather than a GRIN lens, which is possible here only because the target is superficial. +
Included as a non-neuronal preparation: the label is a structural endothelial reporter plus an injected vascular dye, not an activity sensor. Lens implanted immediately after injury in the same procedure. +
Two-photon rather than one-photon, which is why dentate gyrus at over 620 um depth is reachable. Frame rate is correspondingly low (around 8 Hz) compared with the 30 Hz typical of one-photon entries in this registry. +