Experiment/guo 2023 mouse dorsal cortex headfixed

Head-fixed mouse dorsal cortex imaging with Miniscope-LFOV

Bilateral dorsal cortex imaged through a large cranial window in head-fixed mice on a treadmill.

From Publication/guo 2023 miniscopelfov

Extracted from full textTranscribed by a curator from the published methods section. Values not stated in the paper are marked as not reported rather than inferred.

Animal

Species

Mouse

Strain

C57BL/6N Tac

Sex

Male

Age at surgery

3-5 months

Animals

2

Target

Brain region

Dorsal cortex

Coordinates

AP -1.7 and -2.3 from bregma, ML 0.5, DV -0.8

Hemisphere

Bilateral

Indicator

Indicator

GCaMP6f

Reports

Calcium

Delivery

AAV injection

Construct

AAV1.Syn.GCaMP6f.WPRE.SV40

Injection volume

500 nL per site

Optical access

Method

Cranial window

Lens

4 x 4 mm custom coverslip

Surgery staging

Single surgery

Implant to baseplating

3 weeks

Imaging

Scope

MiniLFOV

Condition

Head-fixed

Frame rate

23 fps

Behavior

Head-fixed locomotion on a 29 mm circular treadmill

Analysis

CaImAn, CNMF-E

Cell yield

1,907 neurons detected

Who to ask

Lab PI

Daniel Aharoni

Institution

University of California, Los Angeles

Resources

Data

Notes

Field of view 3.6 x 2.7 mm. Head-fixed rather than freely behaving, and a cranial window rather than a lens — the same scope covering a very different preparation from the two rat entries on this paper.