Experiment/choi 2023 mouse prelimbic
From Miniscope
Mouse prelimbic cortex imaging of striatum-projecting populations
Projection-defined prelimbic imaging: a retrograde Cre virus in dorsomedial striatum gates a Cre-dependent indicator in PL.
Extracted from full textTranscribed by a curator from the published methods section. Values not stated in the paper are marked as not reported rather than inferred.
Animal
Species
Mouse
Strain
C57BL/6NCrl
Transgenic line
B6.FVB(Cg)-Tg(Adora2a-cre)KG139Gsat/Mmcd
Sex
Male
Animals
17
Target
Brain region
Coordinates
AP +2.0, ML +0.35, DV -1.7 from bregma
Hemisphere
Bilateral
Cell type
Striatum-projecting prelimbic pyramidal neurons
Indicator
Indicator
jGCaMP7f
Reports
Calcium
Delivery
Retrograde or trans-synaptic virus
Construct
retroAAV2-EF1a::3XFLAG-Cre (striatum); AAV1-hSyn::FLEX-jGCaMP7f (prelimbic)
Optical access
Method
GRIN lens
Surgery staging
Single surgery
Imaging
Condition
Freely behaving
Sessions
45 min sessions
Behavior
Two-alternative forced choice with feedback
Analysis
MIN1PIPE
Cell yield
274 PL-to-anterior-DMS neurons; 485 PL-to-posterior-DMS neurons
Who to ask
Experimenter
Marc V. Fuccillo
Institution
University of Pennsylvania
Notes
An intersectional strategy: the recorded population is defined by where it projects, not by a genetic marker.