Experiment/keinath 2022 mouse dorsal ca1
From Miniscope
Mouse dorsal CA1 imaging across a geometric morph sequence
Chronic CA1 imaging across six environments over 32 days to track representational drift against geometric change.
Extracted from full textTranscribed by a curator from the published methods section. Values not stated in the paper are marked as not reported rather than inferred.
Animal
Species
Mouse
Strain
C57BL/6
Sex
Male
Age at surgery
6-10 weeks
Animals
5
Target
Brain region
Coordinates
AP -2.1, ML 2.0, DV -1.35 from brain surface
Hemisphere
Not reported
Cell type
CA1 pyramidal neurons
Indicator
Indicator
GCaMP6f
Reports
Calcium
Delivery
AAV injection
Construct
AAV5.CaMKII.GCaMP6f.WPRE.SV40; AAV9.syn.GCaMP6f.WPRE.SV40
Titer
3.26 x 10^14 GC/mL (AAV9); 2.3 x 10^13 GC/mL (AAV5)
Injection volume
350 nL
Expression time
2-6 weeks to imaging
Optical access
Method
GRIN lens
Lens
1.8 mm or 0.5 mm diameter (Go!Foton)
Implant depth
-1.35 mm
Surgery staging
Separate surgeries
Injection to implant
1-3 weeks
Implant to baseplating
1-3 weeks
Imaging
Scope
Condition
Freely behaving
Frame rate
30 Hz
Sessions
One 20 min session per day, one environment per day, across 32 days
Behavior
Geometric morph sequence across six environments
Analysis
CNMF-E, MATLAB R2021a, custom
Cell yield
4,070 cells total; 1,647 tracked across 16 or more sessions
Who to ask
Experimenter
Alexandra T. Keinath
Institution
McGill University
Notes
Three separate surgeries: viral injection, lens implant, then baseplate.