Experiment/blair 2023 rat dorsal ca1
From Miniscope
Rat dorsal CA1 place cell imaging with MiniLFOV
Chronic large-field-of-view imaging of rat dorsal CA1 across a spatial alternation and footshock avoidance protocol.
Extracted from full textTranscribed by a curator from the published methods section. Values not stated in the paper are marked as not reported rather than inferred.
Animal
Species
Rat
Strain
Long-Evans
Sex
Both
Age at surgery
3 months at acquisition (Charles River)
Animals
15
Target
Brain region
Coordinates
AP -3.6, ML 2.5, DV 2.6
Hemisphere
Counterbalanced
Cell type
CA1 pyramidal neurons
Indicator
Indicator
GCaMP7s
Reports
Calcium
Delivery
AAV injection
Construct
AAV9-Syn-GCaMP7s (Addgene)
Injection volume
1.2 uL at 0.12 uL/min
Expression time
~3-4 weeks to baseplating (1 week injection to lens, then 2-3 weeks to baseplate)
Optical access
Method
GRIN lens
Lens
1.8 mm diameter (Edmund Optics); two 0.25-pitch GRIN lenses relayed to 0.5 pitch
Surgery staging
Separate surgeries
Injection to implant
1 week
Implant to baseplating
2-3 weeks
Imaging
Scope
Condition
Freely behaving
Frame rate
22 Hz
Behavior
Rectangular maze spatial alternation, Footshock avoidance
Analysis
CaImAn, CellReg
Who to ask
Experimenter
Hugh T. Blair
Institution
University of California, Los Angeles
Notes
Field of view 3.6 x 2.3 mm. Hemisphere was balanced across animals by design rather than fixed.