Experiment/guo 2023 rat dorsal ca1 lfov

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Rat dorsal CA1 imaging with Miniscope-LFOV

Large-field-of-view CA1 imaging in rats running a spatial alternation task, the wired configuration of the MiniLFOV validation set.
From Publication/guo 2023 miniscopelfov
Extracted from full textTranscribed by a curator from the published methods section. Values not stated in the paper are marked as not reported rather than inferred.
Animal
Species
Rat
Strain
Long-Evans


Age at surgery
3 months
Animals
15
Target
Brain region
Dorsal CA1
Coordinates
AP -3.6, ML 2.5, DV 2.6
Hemisphere
Counterbalanced
Cell type
CA1 pyramidal neurons
Indicator
Indicator
GCaMP7s
Reports
Calcium
Delivery
AAV injection
Construct
AAV9-Syn-GCaMP7s
Injection volume
1.2 uL
Optical access
Method
GRIN lens
Lens
1.8 mm diameter relay GRIN lens
Surgery staging
Separate surgeries
Implant to baseplating
2-3 weeks
Imaging
Scope
MiniLFOV
Condition
Freely behaving
Frame rate
23 fps
Sessions
15 min sessions every 48 hours
Behavior
Linear alternation on a 2.5 x 1.25 m rectangular track
Analysis
CaImAn, CNMF-E, OASIS
Cell yield
1,357 cells detected; 626 place cells (46.2%)
Who to ask
Lab PI
Daniel Aharoni
Institution
University of California, Los Angeles
Resources
Data
Notes
Field of view ~2.54 mm2. Same rat CA1 preparation later used in Blair et al. 2023.
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