Experiment/dong 2025 mouse dorsal ca1 dual color
From Miniscope
Mouse dorsal CA1 two-color imaging with a static reference channel
Simultaneous GCaMP6f and nuclear dTomato imaging on a dual-channel miniscope, using the static red channel as a registration reference for chronic cell tracking.
Extracted from full textTranscribed by a curator from the published methods section. Values not stated in the paper are marked as not reported rather than inferred.
Animal
Species
Mouse
Strain
C57BL/6J
Sex
Male
Animals
9
Target
Brain region
Coordinates
AP -2.0, ML +1.8 from bregma, DV -1.6 from skull surface
Hemisphere
Not reported
Cell type
CA1 pyramidal neurons
Indicator
Indicator
GCaMP6f, nls-dTomato
Reports
Calcium, Structural
Delivery
AAV injection
Construct
AAV1-hSyn-GCaMP6f-P2A-nls-dTomato
Titer
2 x 10^13 genome copies/mL
Injection volume
500 nL at 2 nL/s
Expression time
3 weeks
Optical access
Method
GRIN lens
Lens
1 mm diameter x 4.0 mm, 0.5 pitch (Inscopix)
Implant depth
-1.35 mm from bregma
Surgery staging
Single surgery
Implant to baseplating
3 weeks
Imaging
Condition
Freely behaving
Frame rate
30 fps per channel
Sessions
Seven 15 min sessions at 2-day intervals across 13 days
Behavior
Linear track (1 m) for water reward, Restaurant Row
Analysis
Minian, CNMF, ezTrack, custom Python
Cell yield
~150 cells per session per channel; 46.4% trackable across all seven sessions via dTomato
Who to ask
Experimenter
Denise J. Cai
Lab PI
Institution
Icahn School of Medicine at Mount Sinai
Resources
Notes
10 animals implanted, 9 analyzed. The second colour is a static structural label, not a second activity sensor — its purpose is to make cross-session cell identity verifiable rather than inferred from activity.