Experiment/el-kalliny 2026 vole nac
From Miniscope
"Prairie vole" is not in the list (Mouse, Rat, Non-human primate, Bat, Songbird, Other) of allowed values for the "Has species" property.
Prairie vole nucleus accumbens imaging with local CP-AMPAR pharmacology during pair bond formation
NAc imaging through a GRIN lens with an angled infusion cannula aimed at the field of view, recording during mate cohabitation and partner preference tests after local vehicle or IEM-1460 (CP-AMPAR antagonist) infusion.
Extracted from full textTranscribed by a curator from the published methods section. Values not stated in the paper are marked as not reported rather than inferred.
Animal
Species
Prairie vole
Sex
Both
Age at surgery
60-100 days at surgery
Target
Brain region
Coordinates
Lens AP +1.6, ML +1.05, DV -4.4 mm; virus DV -4.3 to -4.7 mm
Hemisphere
Not reported
Indicator
Indicator
GCaMP6f
Reports
Calcium
Delivery
AAV injection
Construct
AAV1-hSyn-GCaMP6f (Addgene #100837)
Titer
7.4 x 10^12
Injection volume
500 nL (either 100 nL at each of five depths from -4.7 to -4.3 mm DV, or a single 500 nL injection at -4.5 mm DV)
Expression time
At least 4 weeks recovery after surgery for viral expression
Optical access
Method
GRIN lens
Lens
1.0 mm diameter, 9.0 mm length GRIN lens
Implant depth
4.4 mm DV
Surgery staging
Single surgery
Implant to baseplating
At least 3 weeks
Imaging
Scope
Condition
Freely behaving
Frame rate
30 Hz
Sessions
First cumulative hour of cohabitation (Day 0), eighth cumulative hour of cohabitation (Day 1), first hour of partner preference test (Day 2), and a further partner preference test on Day 15; infusions before each cohabitation session only
Behavior
Opposite-sex cohabitation, Partner preference test
Analysis
CaImAn, NoRMCorre, CNMF-E, CellReg, PCA-ICA
Cell yield
45.1 +/- 4.87 neurons per animal; about 45% of neurons co-registered between days
Who to ask
Experimenter
Zoe R. Donaldson
Institution
University of Colorado Boulder
Notes
A unilateral 26-gauge guide cannula was implanted at a 30 degree angle (AP +1.6, ML -2.65, 2.30 mm DV) in the same surgery so vehicle (ACSF) or IEM-1460 (1 mM, 500 nL) could be infused to the imaged neurons; unilateral infusion was chosen because it left partner preference behaviour intact. No tissue aspiration; a blunt 30-gauge needle tract to -3.6 mm DV preceded slow lens lowering to -4.4 mm over 10 min. Lens protected with Body Double silicone until baseplating. Open Ephys V4 Miniscopes and baseplates, overhead MiniCAM, Miniscope DAQ 3.3 over coaxial cable, Miniscope-DAQ-QT Software v1.11, gain 3.5; cables looped well above the chamber to minimise experimenter intervention and untangling; no commutator mentioned. Animals habituated to the scope for 15 min on three days. Videos downsampled to 15 Hz before analysis. Three animals were lost to scope PCB failures and two to lens/cannula mistargeting. Females were estrogen-primed before mate introduction. Lens placement mapped onto mouse atlas sections; field of view spans medial NAc core and shell. Neuropixels 2.0 recordings (females only) and slice patch-clamp in the same paper are not miniscope experiments.