Experiment/hart 2020 rat acc
From Miniscope
Rat anterior cingulate cortex imaging during effort-based choice
Bilateral rat ACC imaging combined with chemogenetic manipulation during progressive-ratio lever pressing.
Extracted from full textTranscribed by a curator from the published methods section. Values not stated in the paper are marked as not reported rather than inferred.
Animal
Species
Rat
Strain
Long-Evans
Sex
Male
Age at surgery
Postnatal day 60 at arrival
Animals
4
Target
Brain region
Coordinates
AP +2.0, ML +/-0.7, DV -1.4 and -0.9 from bregma (two boluses)
Hemisphere
Bilateral
Indicator
Indicator
GCaMP6f
Reports
Calcium
Delivery
AAV injection
Construct
AAV9-CaMKIIa-GCaMP6f
Injection volume
0.5 uL per site, two sites
Expression time
~8 days to first imaging (3 days injection to lens, 5 days lens to imaging)
Optical access
Method
GRIN lens
Lens
1.8 mm diameter, 0.25 pitch (Edmund Optics part 64-519)
Implant depth
2.0 mm ventral from skull surface
Surgery staging
Separate surgeries
Injection to implant
3 days
Implant to baseplating
2-3 weeks
Imaging
Condition
Freely behaving
Frame rate
30 Hz at 480 x 752 px, downsampled to 7.5 fps for analysis
Behavior
Progressive ratio lever pressing with and without freely available chow
Analysis
CNMF, CellReg, custom MATLAB
Cell yield
1,151 neurons total; 227 met inclusion criteria
Who to ask
Experimenter
Alicia Izquierdo
Institution
University of California, Los Angeles
Notes
A notably short 3-day injection-to-implant interval, at the fast end of anything in this registry. Combined with chemogenetic (DREADD) manipulation.