Experiment/jiang 2026 mouse acc
From Miniscope
Paired mouse anterior cingulate cortex imaging during a mutual cooperation task
Simultaneous ACC imaging in both mice of a pair while they coordinated nose pokes within a shared time window for mutual reward.
Extracted from full textTranscribed by a curator from the published methods section. Values not stated in the paper are marked as not reported rather than inferred.
Animal
Species
Mouse
Target
Brain region
Coordinates
injection AP +1.0, ML +0.5, DV -1.8; lens AP +1.0, ML +0.5, DV -1.6 from bregma
Hemisphere
Not reported
Indicator
Indicator
GCaMP7f
Reports
Calcium
Delivery
AAV injection
Construct
AAV5-hSyn-GCaMP7f-mCherry (Addgene)
Injection volume
300 nL at 30 nL/min
Optical access
Method
GRIN lens
Lens
1.0 mm diameter x 4.0 mm length (Inscopix)
Implant depth
DV -1.6 from bregma, above the injection site
Surgery staging
Separate surgeries
Injection to implant
5 days
Imaging
Scope
Condition
Freely behaving
Frame rate
30 fps
Sessions
Sessions of at least 10 min extending to 60 min, with 20-70 trials per session
Behavior
Mutual cooperation nose-poke task, Opaque-divider control
Analysis
NoRMCorre, ImageJ bandpass
Cell yield
12,798 neurons across 17 pairs of mice
Who to ask
Experimenter
Weizhe Hong, Jonathan C. Kao
Institution
University of California, Los Angeles
Notes
Both animals of a pair were imaged at once, which is the unusual feature of this preparation -- two scopes running simultaneously in a shared arena. Imaging was performed after the pair had learned the task. Behaviour recorded at 15 fps on a FLIR infrared camera and annotated with the Hong lab Behavior Annotator.