Experiment/etter 2023 mouse ca1 optogenetics
From Miniscope
Mouse dorsal CA1 imaging with concurrent septal optogenetic stimulation
CA1 imaging while medial septal theta is optogenetically scrambled or paced, combining a GRIN lens with a separate stimulating fiber.
Extracted from full textTranscribed by a curator from the published methods section. Values not stated in the paper are marked as not reported rather than inferred.
Animal
Species
Mouse
Transgenic line
B6;129P2 PV-Cre (JAX 017320)
Sex
Both
Age at surgery
8-16 weeks
Animals
5
Target
Brain region
Coordinates
AP -1.86 from bregma, ML 1.5, DV 1.2 (GRIN lens placement)
Hemisphere
Right
Cell type
CA1 pyramidal neurons
Indicator
Indicator
GCaMP6f
Reports
Calcium
Delivery
AAV injection
Construct
AAV5.CamKII.GCaMP6f.WPRE.SV40 (Addgene 100834)
Injection volume
200 nL at 1 nL/s
Expression time
4 weeks to baseplating
Optical access
Method
GRIN lens
Lens
1.8 mm diameter (Edmund Optics)
Implant depth
DV 1.2 mm
Surgery staging
Separate surgeries
Injection to implant
2 weeks
Implant to baseplating
4 weeks
Imaging
Scope
Condition
Freely behaving
Frame rate
30 Hz
Sessions
15 min recording sessions
Behavior
Three-tone cued linear track, Open field
Analysis
CNMF-E, custom Bayesian decoder
Cell yield
1,849 cells across 5 mice during open field
Who to ask
Experimenter
Guillaume Etter
Institution
McGill University; Douglas Mental Health University Institute
Notes
ChrimsonR stimulation of medial septum delivered through a separate 200 um fiber (5 ms pulses, scrambled or 8 Hz paced) while imaging CA1 — a worked example of combining a miniscope with an independent optogenetic implant.