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Guides
Tutorials, assembly guides, and how-tos contributed by the community.
29
Guides
12
Distinct topics
List
What's Inside Your Miniscope V4 Reference
Jul 2026 · Beginner
Samara scratchpad, A plain-language walkthrough of what each part of an assembled Miniscope V4 does and why, written for researchers imaging animals — not hardware developers.
Hardware, Optics
Miniscope V4 Assembly Protocol
Jun 2026 · Beginner
A walkthrough of assembling an Miniscope V4
Electronics, Optics, Mechanics
Jun 2026 · Beginner
Jun 2026
This is a tutorial guide for the assembly of interface PCB for the UCLA 2P Miniscope
Jun 2026
Jun 2026
Jun 2026
Jun 2026
Anatomy of a miniscope recording Introduction
Jun 2026 · Beginner
A brief overview of what makes up the noise and signal of a miniscope recording with links to interactive notebooks and more info.
Calcium imaging, Optics, Software, Data processing
Getting Started with Miniscopes Introduction
Jun 2026 · Beginner
May 2026 · Advanced
A walkthrough of what considerations and adjustments need to be made to modify or purchase a Miniscope V4 designed to image something other than GCaMP such as rCaMP or PinkyCaMP
Calcium imaging, Optics, Imaging
May 2026 · Intermediate
Overview of methods to use, test, and validate calcium imaging processing
Data analysis, Data management, Data processing, Calcium imaging
Common Pitfalls and FAQs Troubleshooting
May 2026 · Beginner
A discussion of common issues and where to access additional guides and FAQs on the most reoccurring questions around Miniscopes.
May 2026 · Beginner
Calcium imaging, Optics, Mechanics, Imaging
How (and Why) to Submit an Issue Introduction
May 2026 · Beginner
Miniscope tools are driven by the community. When issues, bugs, or challenges come up, it is extremely helpful to us to know about them. This guide will walk you through the how, and why, of submitting problem/bug/issue to the Miniscope Project.
How to Test and Debug your Miniscope V4 Maintenance
May 2026 · Beginner
A walk through of considerations when first testing a Miniscope V4 and some tips and tricks for debugging common issues that might come up across use.
Electronics, Software, Optics, Mechanics, Imaging
Introduction to Open-Source Contribution and Project Standardization of Testing and Validation Introduction
May 2026 · Intermediate
Miniscope V4 Part Procurement Tutorial
May 2026 · Beginner
Surgery and Baseplating Tutorial
May 2026 · Intermediate
A workflow for cortical and deep region surgery and baseplating
May 2026 · Intermediate
An overview and workflow for thinking through and implementing freely behaving animals experiments
Behavior, Calcium imaging, Software, Imaging
May 2026 · Intermediate
This document walks through the typical analysis pipeline, what the dominant algorithms (especially CNMF-E variants) actually do, what one-photon (1p) data can and cannot tell you, and how to assess single-cell calcium activity quality.
Calcium imaging, Software, Imaging, Data processing
May 2026 · Beginner
A walkthrough of steps to validate the functionality of a newly assembled Miniscope on the bench before moving to animal experiments.
Electronics, Imaging, Software, Optics
Miniscope V3 Surgery and Baseplating Tutorial
Oct 2019 · Intermediate
A basic outline of our procedure for implanting a GRIN lens above the hippocampus for CA1 imaging written for the Miniscope V3.
Animal Behavior Introduction
Nov 2016 · Beginner
Reference procedures for animal and imaging preparation, and experimental setup using legacy Miniscope systems.
Behavior
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