Experiment/hur 2024 mouse acc
From Miniscope
Mouse anterior cingulate cortex imaging during social interaction
ACC imaging with an integrated miniscope/electrophysiology E-Scope during social and object interaction.
Extracted from full textTranscribed by a curator from the published methods section. Values not stated in the paper are marked as not reported rather than inferred.
Animal
Species
Mouse
Strain
C57BL/6J
Sex
Male
Age at surgery
6-8 weeks
Target
Brain region
Coordinates
AP +0.9, ML +0.2, DV -1.3 from bregma
Hemisphere
Left
Indicator
Indicator
GCaMP6f
Reports
Calcium
Delivery
AAV injection
Construct
AAV1-Syn-GCaMP6f-WPRE-SV40
Titer
4.5 x 10^13 GC/mL
Injection volume
300 nL
Expression time
~3 weeks to baseplating
Optical access
Method
GRIN lens
Lens
2 mm objective GRIN (4.79 mm length, 0.25 pitch, 0.50 NA, Grintech) relayed through a 1 mm, 4 mm-long GRIN
Implant depth
0.7 mm DV from skull surface
Surgery staging
Separate surgeries
Injection to implant
1 week
Implant to baseplating
2 weeks
Imaging
Scope
Condition
Freely behaving
Frame rate
~30 Hz
Sessions
1 min baseline, 7 min social or object interaction, 1 min inter-session interval
Behavior
Social interaction, Object interaction
Analysis
NoRMCorre, CNMF-E, Miniscope Analysis
Cell yield
~200 ACC neurons per mouse
Who to ask
Experimenter
Peyman Golshani
Institution
University of California, Los Angeles
Resources
Notes
Miniscope carried an integrated electrophysiology headstage (E-Scope) for simultaneous cerebellar recording.