Experiment/li 2024 mouse motor cortex
From Miniscope
Mouse motor cortex layer 2/3 imaging through a cranial window
Superficial motor cortex imaging during single-pellet reaching, using a cranial window rather than an implanted lens.
Extracted from full textTranscribed by a curator from the published methods section. Values not stated in the paper are marked as not reported rather than inferred.
Animal
Species
Mouse
Strain
C57BL/6
Transgenic line
Cck-/-
Sex
Not reported
Animals
24
Indicator
Indicator
GCaMP6s
Reports
Calcium
Delivery
AAV injection
Construct
AAV-hSyn-CaMKII-GCaMP6s-SV40
Titer
~1 x 10^13 copies/mL
Injection volume
0.6 uL
Expression time
More than 14 days
Optical access
Method
Cranial window
Lens
3 x 3 mm cranial window with cover glass
Implant depth
Window at the cortical surface; imaging plane 400-500 um deep
Surgery staging
Separate surgeries
Implant to baseplating
2-3 weeks
Imaging
Scope
Condition
Freely behaving
Sessions
10 min recordings on days 1 and 6 of training
Behavior
Single pellet reaching
Analysis
NoRMCorre, CNMF-E, MATLAB, Fiji
Who to ask
Experimenter
Kuan Hong Wang
Institution
University of Rochester
Notes
Window placed contralateral to the dominant forelimb. Cohorts: 10 C57BL/6, 7 Cck-/-, 7 wild-type with a CCKBR antagonist. One of the few registry entries using a cranial window rather than a GRIN lens, which is possible here only because the target is superficial.