Experiment/barry 2022 mouse striatum cortex oxycodone
From Miniscope
Mouse dorsolateral striatum and motor cortex imaging under oxycodone
Cre-defined striatal projection neurons and motor cortex imaged before and after acute oxycodone.
Extracted from full textTranscribed by a curator from the published methods section. Values not stated in the paper are marked as not reported rather than inferred.
Animal
Species
Mouse
Transgenic line
D1-Cre, A2A-Cre
Sex
Both
Age at surgery
3-4 months
Animals
10
Target
Brain region
Coordinates
Striatum AP +1.0, ML +/-2.0, DV -3.3; cortex AP +0.5, ML +1.0, DV -0.6 from bregma
Hemisphere
Bilateral
Cell type
D1 receptor-expressing MSNs, A2A receptor-expressing MSNs
Indicator
Indicator
GCaMP6f
Reports
Calcium
Delivery
AAV + transgenic
Construct
Cre-dependent AAV-GCaMP6f (striatum); Syn or CaMKII promoter AAV-GCaMP6f (cortex)
Injection volume
1 uL (striatum); 0.5 uL (cortex)
Expression time
3-4 weeks to baseplating
Optical access
Method
GRIN lens over cannula
Lens
1.8 mm diameter (Edmund Optics)
Surgery staging
Separate surgeries
Injection to implant
2 weeks
Implant to baseplating
1-2 weeks
Imaging
Scope
Condition
Freely behaving
Frame rate
20 Hz
Sessions
Baseline plus 5 min post-injection; analysis at the 15 min timepoint
Behavior
Oxycodone (3 mg/kg IP) versus saline in a cylindrical chamber
Analysis
Minian, Cross-reg, ezTrack, ImageJ
Who to ask
Experimenter
Carlos Cepeda
Institution
University of California, Los Angeles
Notes
Cortical preparation required tissue aspiration to seat the lens. Cohorts: 6 striatal (3 D1-Cre, 3 A2A-Cre), 4 cortical (2 Syn, 2 CaMKII).