Experiment/guo 2023 rat acc wirefree

Rat anterior cingulate cortex wire-free imaging with Miniscope-LFOV

Wire-free CA1-scale imaging in rat ACC, recording to an on-board microSD card with no tether.

From Publication/guo 2023 miniscopelfov

Extracted from full textTranscribed by a curator from the published methods section. Values not stated in the paper are marked as not reported rather than inferred.

Animal

Species

Rat

Age at surgery

3 months

Animals

1

Target

Brain region

Anterior cingulate cortex

Coordinates

AP +2.0, ML 0.7

Hemisphere

Not reported

Indicator

Indicator

GCaMP8m

Reports

Calcium

Delivery

AAV injection

Construct

AAV9-Syn-GCaMP8m

Injection volume

0.6 uL across two depths

Optical access

Method

GRIN lens

Lens

1 mm diameter GRIN lens (Thorlabs G1P11)

Implant depth

2.0 mm ventral to skull surface

Surgery staging

Single surgery

Implant to baseplating

3 weeks

Imaging

Scope

MiniLFOVWire-free Miniscope

Condition

Freely behaving, Wire-free

Frame rate

15 fps

Behavior

Circular open field (80 cm diameter)

Analysis

CaImAn, CNMF-E

Cell yield

575 cells detected; 438 place cells (76%)

Who to ask

Lab PI

Daniel Aharoni

Institution

University of California, Los Angeles

Resources

Data

Notes

Wire-free DAQ weighs 3.5 g and gives roughly 1 hour of battery life; data written to microSD. Field of view 1.4 x 1.4 mm. Single demonstration animal.