Experiment/kim 2019 mouse dorsolateral striatum
From Miniscope
Mouse dorsolateral striatum imaging of parvalbumin interneurons
Cre-dependent imaging of striatal fast-spiking interneurons during a continuous target pursuit task.
Extracted from full textTranscribed by a curator from the published methods section. Values not stated in the paper are marked as not reported rather than inferred.
Animal
Species
Mouse
Transgenic line
PV-Cre
Animals
5
Target
Brain region
Coordinates
AP +0.4, ML +/-2.4, DV -2.3 from bregma
Hemisphere
Not reported
Cell type
Parvalbumin fast-spiking interneurons
Indicator
Indicator
GCaMP6s
Reports
Calcium
Delivery
AAV + transgenic
Construct
AAV-EF1a-DIO-GCaMP6s
Injection volume
500 nL
Expression time
2 weeks to baseplating
Optical access
Method
GRIN lens
Lens
1 mm x 9 mm (Inscopix, n=3); 1.8 mm x 4.2 mm (Grintech, n=2)
Implant depth
Directly above the injection site
Surgery staging
Single surgery
Implant to baseplating
2 weeks
Imaging
Condition
Freely behaving
Behavior
Continuous target pursuit
Analysis
CNMF, Mosaic (Inscopix), custom MATLAB
Cell yield
2-11 fast-spiking interneurons per mouse
What was hard about this preparation
Cell yield is very low by miniscope standards — 2 to 11 interneurons per animal — because the target population is sparse. Anyone planning an interneuron preparation should budget animals against per-animal yield rather than total cell count.
Who to ask
Experimenter
Henry H. Yin
Institution
Duke University
Notes
Two different lens geometries were used across the cohort (Inscopix 1 x 9 mm in three animals, Grintech 1.8 x 4.2 mm in two).