Experiment/cheng 2026 mouse mpfc
From Miniscope
"GCaMP" can not be assigned to a declared number type with value 6.
Mouse medial prefrontal cortex imaging during cooperative foraging
Unilateral mPFC imaging through a GRIN lens in leaders or followers of cooperating mouse pairs, relating single-neuron activity to arrival order, choice and egocentric partner position.
Extracted from full textTranscribed by a curator from the published methods section. Values not stated in the paper are marked as not reported rather than inferred.
Animal
Species
Mouse
Strain
C57BL/6J (Jackson Laboratory)
Age at surgery
2-6 months
Animals
GCaMP6f: 6; jGCaMP8m: 6 in spatial selectivity analyses, 2 in choice and leading-versus-following analyses
Target
Brain region
Coordinates
AP +1.98, ML +0.45, DV -1.70 from bregma (injection site)
Hemisphere
Not reported
Indicator
Indicator
GCaMP6f, jGCaMP8m
Reports
Calcium
Delivery
AAV injection
Construct
AAV9-Syn-GCaMP6f-WPRE-SV40 (Addgene 100837-AAV9) or AAV9-Syn-jGCaMP8m-WPRE (Addgene 162375-AAV9)
Titer
GCaMP6f 7 x 10^12 vg/mL; jGCaMP8m 1 x 10^13 vg/mL
Injection volume
600 nL
Expression time
2-3 weeks to baseplating
Optical access
Method
GRIN lens
Lens
1 mm diameter, 4 mm length GRIN lens (Inscopix)
Implant depth
1.45 mm below the brain surface
Surgery staging
Single surgery
Implant to baseplating
2-3 weeks
Imaging
Condition
Freely behaving
Sessions
One mouse of the pair imaged per session (leader or follower) during well-trained stage 4a while the partner wore a weight-matched dummy scope; 4-11 sessions per animal
Behavior
Cooperative foraging, Free social interaction outside the task, Mismatch-rule retraining
Analysis
Suite2p, Cascade
Who to ask
Experimenter
Herbert Zheng Wu
Institution
Icahn School of Medicine at Mount Sinai
Notes
Scope cited only as "a Miniscope" (Cai et al. 2016) acquired with the Miniscope DAQ system; no version stated. Tissue above the mPFC was aspirated through a 1 mm radius craniotomy before lens implantation; lens sealed with Kwik-Sil and dental acrylic, and baseplate covered with black dental cement plus a screw-on protective cap. A TTL from the Miniscope DAQ triggered the FLIR behaviour camera (30 fps) through a 6-pin GPIO Hirose cable for frame-by-frame alignment. Delta F/F used 70% neuropil subtraction and a dynamic baseline. Imaging was done primarily in female pairs because post-surgical males could not be reliably re-paired; sex of the imaged animals is not broken down. GCaMP6f and jGCaMP8m animals were pooled in analyses.