Miniscope V4

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Active

Sub-project of UCLA Miniscope Project

The landing page for the open-source UCLA Miniscope V4 project

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Specifications

No specifications yet — declare them under specs: in the project root wiki.yml and they'll appear after the next tagged release.

Components

No components in the latest release yet.

Releases

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Posts

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Guides

• Guide/Cranial window surgeries (Description: Overview surgery protocol describing the placement of a cranial window over the dorsal cortex.)
• Guide/Miniscope V4 Assembly (Description: A walkthrough of assembling an Miniscope V4)
• Guide/Getting started with miniscopes
• Guide/Converting a Miniscope V4 for a different fluorophore (Description: A walkthrough of what considerations and adjustments need to be made to modify or purchase a Miniscope V4 designed to image something other than GCaMP such as rCaMP or PinkyCaMP)
• Guide/Data processing considerations (Description: This document walks through the typical analysis pipeline, what the dominant algorithms (especially CNMF-E variants) actually do, what one-photon (1p) data can and cannot tell you, and how to assess single-cell calcium activity quality.)
• Guide/How to test and debug your Miniscope V4 (Description: A walk through of considerations when first testing a Miniscope V4 and some tips and tricks for debugging common issues that might come up across use.)
• Guide/Miniscope V4 part procurement (Description: Intro guide on how to purchase V4 Miniscopes and Miniscope components.)
• Guide/A test suite for working with miniscope data (Description: Overview of methods to use, test, and validate calcium imaging processing)
• Guide/Surgery and baseplating (Description: A workflow for cortical and deep region surgery and baseplating)
• Guide/Initial testing of assembled miniscope (Description: A walkthrough of steps to validate the functionality of a newly assembled Miniscope on the bench before moving to animal experiments.)

FAQs

• FAQ/Banding from ground loops and shared power (Question: I get banding that moves up the sensor even at low LED values, and I am the only one in the lab with the problem. Why?)
• FAQ/Difference between Miniscope V3 and V4 (Question: What's the difference between Miniscope V3 and Miniscope V4?)
• FAQ/Different between V4 4 and V4 5 (Question: What's different between V4.4 and V4.5?)
• FAQ/Horizontal stripes from the EWL driver (Question: I get fine horizontal stripes across the image, worst when gain is high. Where do they come from?)
• FAQ/Objective versus relay GRIN lenses (Question: I see nothing at all at baseplating, even though the optics, expression and aspiration depth all check out. What did I miss?)
• FAQ/Shorted coax cable and the L13 inductor (Question: My DAQ suddenly stopped working and now every DAQ I plug this cable into fails too. What happened?)
• FAQ/Where to buy V4 (Question: Where can I buy a Miniscope V4?)

Publications

• Publication/jiang 2026 cooperative behavior biological artificial (Year: 2,026)
• Publication/chapman 2026 piriform odor representation (Year: 2,026)
• Publication/el-kalliny 2026 accumbal calcium permeable ampa receptors (Year: 2,026)
• Publication/skromne carrasco 2026 monthslong (Year: 2,026)
• Publication/park 2026 offline engram reverberation amygdala (Year: 2,026)
• Publication/sun 2026 shared substrates prosocial parenting (Year: 2,026)
• Publication/raam 2026 cortical regulation collective social dynamics (Year: 2,026)
• Publication/dong 2025 simultaneous (Year: 2,025)
• Publication/asaoka 2025 dissociable (Year: 2,025)
• Publication/beacher 2025 miniscope (Year: 2,025)
• Publication/park 2025 hippocampal cortical consolidation social memory (Year: 2,025)
• Publication/lin 2024 hippocampal (Year: 2,024)
• Publication/mishchanchuk 2024 hidden (Year: 2,024)
• Publication/chen 2024 decoding (Year: 2,024)
• Publication/borkar 2024 topdown (Year: 2,024)
• Publication/raymond 2024 neural (Year: 2,024)
• Publication/carrasco 2024 monthslong (Year: 2,024)
• Publication/simoes de souza 2024 miniscope (Year: 2,024)
• Publication/sun 2024 hippocampal (Year: 2,024)
• Publication/wang 2024 tscope (Year: 2,024)
• Publication/froula 2024 distinct (Year: 2,024)
• Publication/jendryka 2024 excitatory (Year: 2,024)
• Publication/li 2024 cholecystokinin (Year: 2,024)
• Publication/zhang 2024 cortical (Year: 2,024)
• Publication/celinskis 2024 toward (Year: 2,024)

... further results

SOPs

No SOPs reference this project yet.