Miniscope Zero/Component/Miniscope Zero Optics/0.2.10

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Miniscope Zero Optics 0.2.10

Hardware Component
Description Folded optical path of the miniscope: excitation and emission optics, 3D-printed housing, and CNC-machined baseplate.
Design File URL https://github.com/miniscope/MiniscopeZero/tree/v0.1.1/MiniscopeZero/Optics
Hardware Type Optical


Name Miniscope Zero Optics
Project Miniscope Zero


Source Path MiniscopeZero/Optics
Version 0.2.10
Backlinks
Has component Miniscope Zero/Release/0.1.1

Specifications

NameValueUnitNotes
Field of View600 × 600um
Resolution6.2um



Design Files

README

Optics

Optical system of the Miniscope Zero head-mounted assembly: bill of materials, mechanical drawing, optical design files, and housing/baseplate CAD.

Contents

Optics/
├── optics_bom.csv    Bill of materials for the optical path
├── mechanical/       Dimensioned drawing of the optical assembly (Fusion 360 export)
├── zemax/            Ansys Zemax OpticStudio sequential-mode design files
├── housing/          3D-printed optical housing CAD
└── baseplate/        3D-printed baseplate CAD and hardware list

The housing holds the optical stack; the baseplate is cemented to the skull and receives it. A set-screw mechanism sets the focal plane relative to the implanted GRIN lens during baseplating, then locks it for subsequent recordings.

Assembly

guides/optics_assembly.md is the build guide for the head-mounted assembly: which optic goes into which printed part and in which orientation, edge blackening and adhesive practice, the order the four printed parts are joined in, and a dark-field leak test to run before the scope goes on an animal.

Bill of materials

optics_bom.csv lists every element in the optical path. GitHub renders it as a table in the web UI. The ref column keys each part to the mechanical drawing:

ref Element
LED Excitation LED
F_ex Excitation filter
DM Dichroic beamsplitter
L1, L2 Objective (L2 is closest to the sample)
L3 Tube lens
M1 Fold mirror
F_em Emission filter
IS Image sensor

Light path: LED → F_ex → DM (transmitted) → L1 → L2 → sample, then sample → L2 → L1 → DM (reflected 90°) → L3 → M1 (folded) → F_em → IS.

Folding the path twice distributes the elements laterally rather than vertically, keeping the assembly height low.

The notes column records where a part is modified from stock — the filters, dichroic, and mirror are all diced down from larger catalog parts. Ordering the stock part number alone will not give you a part that fits the housing.

Links in the link column point to the vendor part page, or to a vendor search for the stock number where no stable product URL exists.

Mechanical drawing

mechanical/drawing_Miniscope_Wireless_emission_v_0_2_10.pdf — dimensioned layout of the optical stack, exported from Fusion 360 (design v0.2.10), with element spacings in mm.

Housing

housing/ holds the 3D-printed optical housing (design v0.2.10):

  • Wireless_Miniscope_v_0_2_10.step — all four bodies as exact solids. Use this to modify the design in any CAD package.
  • Wireless_Miniscope_v_0_2_10.f3d — Fusion 360 archive with the design history.
  • One STL per body, for printing:
File Body Size (mm)
base_unit.stl Main chassis, tube-lens bore 11.3 x 13.0 x 9.0
objective_unit.stl Objective lens holder 7.9 x 2.1 x 7.9
excitation_unit.stl Excitation LED and filter arm 7.6 x 7.6 x 9.0
focus_slider_unit.stl Set-screw focus slider 8.4 x 10.0 x 8.0

The four parts print separately. Each STL is a single watertight, manifold shell at the origin, so it can be oriented independently in the slicer.

Printed on a Formlabs Form 4 in Black Resin V5 at 25 um layer thickness.

Baseplate and dust cap

baseplate/ holds the skull-mounted baseplate and its dust cap. The two are made differently:

Part Process File to use
Baseplate CNC machined (Protolabs) baseplate.step
Dust cap 3D printed dust_cap.stl

The dust cap prints on the same settings as the housing: Formlabs Form 4, Black Resin V5, 25 um layer thickness.

Baseplate machining specification (as ordered from Protolabs):

Material Aluminium 7075-T651
Process Mill
Dimensions 14.59 x 5.99 x 14.89 mm
Tolerance +/- 0.005 in (0.13 mm)
Edges Broken; tool marks visible
Internal corners Sharp, at minimum tool radius
Threading 1 feature, UNF #2-64

The threaded bore is highlighted in green below. It takes the set screw that locks the optical stack in the baseplate once the focal plane is set.

Baseplate seen from above, with the UNF #2-64 threaded bore highlighted in green

baseplate.step is the exact solid sent for machining; baseplate.stl is the tessellated equivalent, for viewing and fit checks. baseplate_and_dustcap.f3z is the Fusion 360 assembly archive for both parts.

baseplate/baseplate_components.csv lists the hardware that goes with it: the McMaster-Carr 92311A317 set screw (18-8 stainless, cup tip, 2-64 UNF x 1/8 in, 0.035 in / 0.9 mm hex drive) and the precision hex driver used to turn it.

Optical design

zemax/ contains the sequential-mode OpticStudio models:

  • Miniscope_Wireless_emission_v_0_2_6_mirror_as_prism_2ndaspheric_14572.zmx — emission path (the fold mirror is modelled as a prism).
  • Miniscope_Wireless_excitation_v_0_2_2.zmx — excitation path.
  • emission_prescription_data.txt — prescription dump of the emission model (surface data, NA, working F/#, track length), readable without OpticStudio.

.ZDA files are the matching OpticStudio session/analysis files.