Questions on calcium imaging in Marmoset using Miniscope LFOV
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Questions on calcium imaging in Marmoset using Miniscope LFOV
Hi all,
Our lab is interested in exploring whether Miniscope LFOV model could be adapted for optical imaging in marmoset V4. I had a few questions about possible design modifications.
First, given that V4 is relatively superficial compared with many other brain regions, Would it be feasible to use a cranial window with cover glass instead of a GRIN lens?
Second, since we work with marmosets and want to make the most out of each animal, we are interested in a design that would allow us to reposition the miniscope across a larger craniotomy. To my understanding, the Miniscope LFOV has a maximum field of view of approximately 3.6 × 2.7 mm. If we were to make, for example, a 5 × 5 mm craniotomy, we would ideally like a design that allows us to shift the miniscope laterally within that window. Does anyone have experience in working with these kind of problems? Justin Zheng (talk) 21:29, 1 June 2026 (PDT)