Experiment/van der veldt 2021 mouse lateral septum
From Miniscope
Mouse lateral septum imaging of GABAergic neurons
VGAT-Cre lateral septum imaging along the anterior-posterior axis, with dorsal CA1 and CA3 imaged in the same study for comparison.
Extracted from full textTranscribed by a curator from the published methods section. Values not stated in the paper are marked as not reported rather than inferred.
Animal
Species
Mouse
Strain
C57BL/6J
Transgenic line
VGAT-IRES-Cre, Grik4-Cre
Sex
Both
Age at surgery
8-16 weeks
Animals
54
Target
Brain region
Coordinates
LS anterior AP +0.86, ML 0.35, DV -3.0; LS intermediate AP +0.38, ML 0.35, DV -2.7; LS posterior AP +0.10, ML 0.35, DV -2.5; CA1 AP -1.8, ML 1.5, DV -1.5; CA3 AP -2.1, ML 2.3, DV -2.2 from bregma
Hemisphere
Not reported
Cell type
GABAergic (VGAT-expressing) septal neurons
Indicator
Indicator
GCaMP6f
Reports
Calcium
Delivery
AAV + transgenic
Construct
AAV2/9.Syn.Flex.GCaMP6f.WPRE.SV40 (LS); AAV5.CamKII.GCaMP6f.WPRE.SV40 (CA1/CA3)
Expression time
4-6 weeks
Optical access
Method
GRIN lens
Lens
0.5 mm diameter (lateral septum); 1.8 mm diameter (CA1)
Surgery staging
Separate surgeries
Injection to implant
2-4 weeks
Implant to baseplating
~4 weeks (CA1)
Imaging
Condition
Freely behaving
Frame rate
30 Hz
Sessions
One 15 min recording session per day
Behavior
Linear track (100-130 cm), Open field (45x45 or 49x49 cm), T-maze non-match-to-place
Analysis
CNMF-E, DeepLabCut, Miniscope Analysis
Cell yield
~2,000 lateral septum neurons across cohorts
Who to ask
Experimenter
Sylvain Williams
Institution
McGill University; Douglas Mental Health University Institute
Resources
Notes
Cohorts: 15 LS animals on linear track, 28 LS in open field, 5 CA1, 6 CA3. The 0.5 mm lens is dictated by how narrow the lateral septum is next to the midline ventricles.