Experiment/sun 2026 mouse mpoa
From Miniscope
Mouse medial preoptic area imaging during prosocial and parenting behaviour
MPOA imaging while mice interacted with unstressed adults, stressed adults and pups, comparing prosocial allogrooming against parenting.
Extracted from full textTranscribed by a curator from the published methods section. Values not stated in the paper are marked as not reported rather than inferred.
Animal
Species
Mouse
Strain
C57BL/6J (JAX 000664)
Sex
Both
Age at surgery
8-10 weeks at purchase
Target
Brain region
Coordinates
AP +0.10, ML +0.45, DV -5.10 from bregma
Hemisphere
Not reported
Indicator
Indicator
jGCaMP7f
Reports
Calcium
Delivery
AAV injection
Construct
AAV1-Syn-jGCaMP7f
Injection volume
400 nL
Optical access
Method
GRIN lens
Lens
Inscopix GRIN lens
Implant depth
0.20 mm above the virus injection site
Surgery staging
Single surgery
Implant to baseplating
2-3 weeks
Imaging
Scope
Condition
Freely behaving
Frame rate
15 Hz
Sessions
One experiment per animal per day, spanning several interaction sessions with different social targets
Behavior
Interaction with stressed conspecific, Interaction with unstressed conspecific, Pup interaction, Sucrose consumption
Analysis
MiniscopeAnalysis, NoRMCorre, CNMF-E
Cell yield
1,772 neurons imaged in total
Who to ask
Experimenter
Weizhe Hong
Institution
University of California, Los Angeles
Resources
Notes
Lens protected with Kwik-cast silicone adhesive after implantation. Scope controlled through the Aharoni-Lab Miniscope-DAQ-QT-Software with an Open Ephys acquisition board. Fields in Figs 1 and 3 come from the same cohort.