Experiment/raam 2026 mouse dmpfc
From Miniscope
Mouse dorsomedial prefrontal cortex imaging during group huddling under cold stress
dmPFC imaging in one animal of a four-mouse group during a 5 degree C thermal challenge, separating self-initiated from partner-initiated huddling decisions.
Extracted from full textTranscribed by a curator from the published methods section. Values not stated in the paper are marked as not reported rather than inferred.
Animal
Species
Mouse
Sex
Male
Animals
11
Target
Brain region
Coordinates
injection AP +2.0, ML +/-0.35, DV -1.8; lens AP +2.0, ML 0.45, DV -1.62
Hemisphere
Not reported
Indicator
Indicator
GCaMP6f
Reports
Calcium
Delivery
AAV injection
Construct
AAV1-Syn-GCaMP6f
Optical access
Method
GRIN lens
Lens
1.0 mm diameter x 4.0 mm length (Inscopix)
Implant depth
DV -1.62; 1 mm of overlying tissue aspirated with a 28-gauge needle through a 1.1 mm craniotomy
Surgery staging
Separate surgeries
Injection to implant
1 week
Implant to baseplating
3 weeks
Imaging
Scope
Condition
Freely behaving
Frame rate
15 Hz
Sessions
30-45 min huddling sessions; one animal recorded per session in the presence of three cagemates
Behavior
Thermal challenge assay at 5 degrees C, Group huddling
Analysis
ImageJ FFT bandpass, CNMF-E
Cell yield
5,141 dmPFC neurons across 11 animals
What was hard about this preparation
Implanted animals were cohoused in pairs behind a perforated acrylic divider for the three weeks between lens implantation and baseplating, then re-cohoused in groups of four with their original cagemates -- a housing constraint worth planning for in any group-behaviour imaging study.
Who to ask
Experimenter
Weizhe Hong
Institution
University of California, Los Angeles
Notes
Scope purchased from Open Ephys and run on the Aharoni-Lab Miniscope-DAQ-QT-Software. CNMF-E output was manually inspected to drop non-somatic components and merge split ROIs.