Experiment/park 2025 mouse il nacsh vca1 hm4di
From Miniscope
"(8mCherry,9hM4Di)" can not be assigned to a declared number type with value 17.
Mouse infralimbic cortex imaging of nucleus accumbens shell-projecting neurons with offline chemogenetic ventral CA1 inactivation
IL-NAcSh imaging through a GRIN lens-prism assembly during an extended social familiarization/recognition task, with hM4Di silencing of vCA1-IL neurons during the offline consolidation phase.
Extracted from full textTranscribed by a curator from the published methods section. Values not stated in the paper are marked as not reported rather than inferred.
Animal
Species
Mouse
Strain
Wild-type Ai148 mice (as stated)
Sex
Male
Age at surgery
Raised to PND 42 before use
Animals
17 (8 mCherry, 9 hM4Di)
Target
Brain region
Coordinates
AP +2.4, ML ±1.0 from bregma, DV -2.3 from skull (10° angled injection)
Hemisphere
Not reported
Indicator
Indicator
GCaMP6f
Reports
Calcium
Delivery
AAV injection
Construct
1:1 AAVrg-Ef1a-Flpo + AAV2-flex-GCaMP6f into IL; AAVrg-Ef1a-Cre into NAc shell; AAV8-hsyn-fDIO-hM4Di-mCherry or AAV8-hsyn-fDIO-mCherry into vCA1
Titer
5 x 10^12 GC/mL
Injection volume
250 nL (IL), 200 nL (NAc shell), 300 nL (vCA1)
Expression time
2 weeks to lens implantation
Optical access
Method
GRIN lens
Lens
1 mm diameter, 4 mm length GRIN lens (Inscopix) glued to a 1 mm aluminium-coated right-angle prism (Edmund Optics #86-621) with NOA65
Implant depth
2.5 mm
Surgery staging
Separate surgeries
Injection to implant
2 weeks
Implant to baseplating
Baseplate cemented during the lens implantation surgery
Imaging
Scope
Condition
Freely behaving
Frame rate
20-30 fps
Sessions
Familiarization with two novel mice on day 1, CNO during the offline phase, recognition test with two familiar, two novel and two littermate conspecifics the next day
Behavior
Extended social familiarization/recognition task, Chemogenetic inactivation
Analysis
ImageJ, custom-modified CNMF-E
Cell yield
1,038 cells in 8 mCherry mice (454 social); 862 cells in 9 hM4Di mice (361 social)
Who to ask
Experimenter
Yong-Seok Lee
Institution
Seoul National University College of Medicine
Resources
Notes
Intersectional strategy: retrograde Cre from NAc shell drives flex-GCaMP6f in IL-NAcSh neurons, while retrograde Flpo from IL drives fDIO-hM4Di in vCA1-IL neurons. The paper calls these mice wild-type Ai148; whether that means reporter-negative littermates is not spelled out. Lens surgery, prism assembly, commutator and acquisition as in the Fig. 2 preparation. Virus was injected bilaterally; the imaged side is not stated. Co-corresponding author Doyun Lee (Institute for Basic Science). GRIN lens is from Inscopix but the scope is the UCLA Miniscope V4.