Property: Has abstract
From Miniscope
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| Display name | Abstract |
| Description | Full abstract or summary of a publication |
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Imaging large-population, single-cell fluorescent dynamics in freely behaving animals larger than mice remains a key endeavor of neuroscience. We present a large-field-of-view open-source miniature microscope (MiniLFOV) designed for large-scale (3.6 mm × 2.7 mm), cellular resolution neural imaging in freely behaving rats. It has an electrically adjustable working distance of up to 3.5 mm ± 100 μm, incorporates an absolute head orientation sensor, and weighs only 13.9 g. The MiniLFOV is capable of both deep brain and cortical imaging and has been validated in freely behaving rats by simultaneously imaging >1000 GCaMP7s-expressing neurons in the hippocampal CA1 layer and in head-fixed mice by simultaneously imaging ~2000 neurons in the dorsal cortex through a cranial window. The MiniLFOV also supports optional wire-free operation using a novel, wire-free data acquisition expansion board. We expect that this new open-source implementation of the UCLA Miniscope platform will enable researchers to address novel hypotheses concerning brain function in freely behaving animals. A large field of view open-source miniature microscope is capable of imaging large populations of neurons in rats and mice. +
Imaging neural fluorescence dynamics during unconstrained behavior remains a major challenge in neuroscience. Head-mounted miniature one-photon microscopes enable in vivo recordings from freely behaving animals. Still, their power and data requirements typically necessitate tethers or heavy batteries, which constrain experiments and may introduce behavioral artifacts. We present Miniscope Zero, a fully wireless miniature microscope platform combining quasistatic cavity resonance (QSCR) wireless power transfer with a high-bandwidth optical data link. The system delivers >500 mW of receiver power across a 2,500 cm2 behavioral arena and streams imaging data at 8 Mbps, supporting real-time data acquisition. Miniscope Zero provides a typical field of view of 600 μm x 600 μm and a 2.6-fold higher light-collection efficiency than the UCLA Miniscope v4. We demonstrate wireless CA1 GCaMP6f recordings during open-field navigation, enclosed-maze exploration, simultaneous multi-animal imaging, and extended three-dimensional behavior, enabling long-duration cellular-resolution imaging without tether-induced constraints. +
Social animals recognize familiar conspecifics and selectivelyavoid harmful ones. As social relationships shift, continuousupdating of social valence is essential, yet the underlying neuralmechanisms remain unclear. Here, by artificially transforming apreviously neutral conspecific into an aggressive one, we showthat valence updating depends on enhanced synapticconnectivity and physiological changes within the hippocampalventral CA1 (vCA1)–basolateral amygdala (BlA)–nucleusaccumbens (NAc) circuit. Following defeat, social memoryengram neurons in the vCA1 strengthened their connectionswith BlA neurons carrying negative valence. The vCA1–BlA–NAcneural circuit flexibly regulates adaptive social behaviors. +
Capturing the intricate dynamics of neural activity in freely behaving animals is essential for understanding the neural mechanisms underpinning specific behaviors. Miniaturized microscopy enables investigators to track population activity at the cellular level, but the field of view (FOV) of these microscopes has often been limited and do not support multi-brain region imaging. To fill this technological gap, we have developed the eXtra Large FOV Miniscope (MiniXL) for mice, a 3.5-gram miniaturized microscope with an FOV measuring 3.5 mm in diameter. We demonstrate the capabilities of the MiniXL through large-scale neuronal population records in hippocampal dorsal CA1. We also demonstrate simultaneous multi-brain region imaging across bilateral medial prefrontal cortex (mPFC) and mPFC and nucleus accumbens (NAc) during complex social behavior and stably track cells across multiple days. As with all microscopes in the UCLA Miniscope ecosystem, the MiniXL is fully open-source and designed to be shared with the neuroscience community to lower the barriers for adoption of this technology. MiniXL expands open-source neural imaging in freely behaving mice, enabling large-scale, multi-region recordings. +