Experiment/zhao 2025 mouse minixl multiregion
From Miniscope
Mouse multi-region imaging with MiniXL
Simultaneous imaging of two separated targets — bilateral mPFC, or mPFC together with nucleus accumbens — through a single large-field-of-view scope.
Extracted from full textTranscribed by a curator from the published methods section. Values not stated in the paper are marked as not reported rather than inferred.
Animal
Species
Mouse
Strain
C57BL/6J
Sex
Male
Age at surgery
6-7 weeks
Target
Coordinates
dCA1 AP -2.1, ML 2.0, DV -1.65; mPFC AP +1.8, ML 0.4, DV -2.1; NAc AP +1.3, ML 1.0, DV -4.7
Hemisphere
Bilateral
Indicator
Indicator
GCaMP6f
Reports
Calcium
Delivery
AAV injection
Construct
AAV1.Syn.GCaMP6f.WPRE.SV40; retro-Cre; Flex-GCaMP6f
Injection volume
400 nL (dCA1, NAc); 300 nL (bilateral mPFC); 350 nL (Syn.GCaMP6f)
Expression time
5-7 days to lens implant
Optical access
Method
GRIN lens
Lens
1.8 mm diameter x 4.7 mm (dCA1); 1 mm x 4 mm (mPFC); 0.5 mm x 6.1 or 8.4 mm (mPFC, NAc)
Implant depth
dCA1 lens centre AP -2.1, ML 1.8, DV -1.25
Surgery staging
Single surgery
Implant to baseplating
2-3 weeks
Imaging
Scope
Condition
Freely behaving
Frame rate
23 fps
Sessions
Linear track every 2 days for 3 sessions; open field and social sessions single
Behavior
Linear track (2 m), Open field (45x45x30 cm), Social interaction and object exploration
Analysis
CaImAn, CNMF-E, CellReg, MiniscopeAnalysis
Cell yield
1,640 cells across 3 days (dCA1); 764 (open field); ~500 per session (bilateral mPFC)
Who to ask
Experimenter
Peyman Golshani
Lab PI
Institution
University of California, Los Angeles
Notes
3.5 mm diameter field of view is what makes two separated implants imageable at once. Social interaction cohort was N = 3.