Experiment/wang 2021 mouse pmd
From Miniscope
Mouse dorsal premammillary nucleus imaging during escape
Cre-dependent PMd imaging across rat exposure and contextual fear, relating population activity to escape vigor.
Extracted from full textTranscribed by a curator from the published methods section. Values not stated in the paper are marked as not reported rather than inferred.
Animal
Species
Mouse
Transgenic line
Cck-IRES-Cre
Sex
Both
Age at surgery
2-6 months
Animals
9
Target
Brain region
Coordinates
AP -2.46, ML -0.5, DV -5.35 from bregma
Hemisphere
Not reported
Cell type
Cck-expressing PMd neurons
Indicator
Indicator
GCaMP6s
Reports
Calcium
Delivery
AAV + transgenic
Construct
AAV9-DIO-GCaMP6s
Titer
3 x 10^12 particles/mL
Injection volume
0.16 uL
Optical access
Method
GRIN lens
Lens
7 mm GRIN lens
Implant depth
200 um above the infusion site
Surgery staging
Single surgery
Implant to baseplating
3 weeks
Imaging
Condition
Freely behaving
Frame rate
30 Hz
Sessions
Multiple sessions across 11 days
Behavior
Live rat exposure, Contextual fear conditioning
Analysis
CNMF-E, UCLA Miniscope Analysis
Cell yield
466 cells (rat assay); 513 cells (fear retrieval)
Who to ask
Experimenter
Avishek Adhikari
Institution
University of California, Los Angeles
Resources
Notes
Lens deliberately seated 200 um dorsal to the injection site rather than at it.