Experiment/van den boom 2023 mouse multiregion
From Miniscope
Mouse cortico-striatal imaging during deep brain stimulation in a compulsivity model
Six separate cortical and striatal targets imaged across SAPAP3-knockout and wild-type mice with concurrent internal-capsule DBS.
Extracted from full textTranscribed by a curator from the published methods section. Values not stated in the paper are marked as not reported rather than inferred.
Animal
Species
Mouse
Transgenic line
SAPAP3-/-
Sex
Both
Age at surgery
2-8 months (mean ~4 months)
Animals
58
Target
Brain region
Coordinates
lOFC AP +2.8, ML +/-1.5, DV -2.2; mOFC AP +2.6, ML +/-0.5; PL AP +2.1; M2 AP +2.3; DS AP +1.1; VS AP +1.1
Hemisphere
Counterbalanced
Indicator
Indicator
GCaMP6s
Reports
Calcium
Delivery
AAV injection
Construct
AAV-DJ-CaMKIIa-GCaMP6s (cortex); AAV-DJ-hSyn-GCaMP6s (striatum)
Titer
3 x 10^12 vg/mL diluted 1:10 (cortex); 5 x 10^12 vg/mL diluted 1:5 (striatum)
Injection volume
500 nL total as two 250 nL injections at 200 nL/min
Expression time
3 weeks
Optical access
Method
GRIN lens
Lens
0.6 mm diameter, ~7.3 mm long relay lens (Inscopix)
Implant depth
Cortex -2.2 mm; striatum -2.7 to -4.8 mm
Surgery staging
Single surgery
Implant to baseplating
3 weeks
Imaging
Condition
Freely behaving
Sessions
Weekly imaging; four DBS blocks per session, eight trials per block
Behavior
Open field with grooming and locomotion scoring
Analysis
Bonsai, JAABA
Cell yield
~750 neurons total across targets
Who to ask
Experimenter
Ingo Willuhn
Institution
Netherlands Institute for Neuroscience
Notes
Bilateral internal-capsule DBS electrodes were implanted in the same surgery as the unilateral GRIN lens. Six targets were imaged across the cohort, one per animal, not simultaneously; virus titer was deliberately diluted differently for cortex and striatum.