Experiment/suto 2026 mouse nac d1r d2r
From Miniscope
Mouse nucleus accumbens imaging of D1R and D2R neurons during social valence updating
Parallel NAc imaging in D1R-Cre and D2R-Cre mice under the same three-session defeat paradigm used for the BLA recordings.
Extracted from full textTranscribed by a curator from the published methods section. Values not stated in the paper are marked as not reported rather than inferred.
Animal
Species
Mouse
Transgenic line
D1R-Cre, D2R-Cre
Sex
Male
Age at surgery
12-20 weeks (24-30 g)
Target
Brain region
Coordinates
AP +1.42, ML +/-0.60, DV -4.20 from bregma
Hemisphere
Not reported
Cell type
D1 receptor-expressing NAc neurons, D2 receptor-expressing NAc neurons
Indicator
Indicator
jGCaMP8s
Reports
Calcium
Delivery
AAV + transgenic
Construct
AAV9-hSyn:FLEX-jGCaMP8s (Addgene 162377)
Titer
1.4 x 10^13 GC/mL
Injection volume
300 nL at 2-3 nL/s
Expression time
3 weeks to baseplating
Optical access
Method
GRIN lens
Lens
500 um diameter (Go!Foton)
Implant depth
At the injection site
Surgery staging
Single surgery
Implant to baseplating
3 weeks
Imaging
Condition
Freely behaving
Frame rate
20 Hz
Sessions
Three sessions: before familiarisation, after familiarisation, and after defeat; two 5 min trials per session
Behavior
Three-chamber social discrimination test, Social defeat
Analysis
Doric Neuroscience Studio, Mosaic, EXTRACT, OASIS
Who to ask
Experimenter
Teruhiro Okuyama
Institution
Institute for Quantitative Biosciences, The University of Tokyo
Notes
A variant cohort combined this NAc D2R imaging with chemogenetic silencing of the BLA: 120 nL of AAV5-CaMKIIa:hM4D(Gi)-mCherry (Addgene 50477; 9.5 x 10^12 GC/mL) bilaterally in BLA, with DCZ at 0.1 mg/kg given 15 min before imaging. Same virus and lens as the plain condition otherwise.