Experiment/suto 2026 mouse bla rspo2
From Miniscope
Mouse basolateral amygdala imaging of Rspo2 neurons during social valence updating
Cre-dependent BLA imaging across three sessions spanning familiarisation and social defeat, tracking how aggressor representations change.
Extracted from full textTranscribed by a curator from the published methods section. Values not stated in the paper are marked as not reported rather than inferred.
Animal
Species
Mouse
Transgenic line
Rspo2-Cre
Sex
Male
Age at surgery
12-20 weeks (24-30 g)
Target
Brain region
Coordinates
injection AP -1.60, ML +/-3.20, DV -4.90 from bregma; implant AP -1.60, ML +/-3.20
Hemisphere
Not reported
Cell type
Rspo2-expressing BLA neurons
Indicator
Indicator
jGCaMP8s
Reports
Calcium
Delivery
AAV + transgenic
Construct
AAV1-hSyn:FLEX-jGCaMP8s (Addgene 162377)
Titer
6.3 x 10^12 GC/mL
Injection volume
500 nL at 2-3 nL/s
Expression time
3 weeks to baseplating
Optical access
Method
GRIN lens
Lens
500 um diameter (Go!Foton)
Implant depth
At the injection site
Surgery staging
Single surgery
Implant to baseplating
3 weeks
Imaging
Condition
Freely behaving
Frame rate
20 Hz
Sessions
Three sessions: before familiarisation, after familiarisation, and after defeat; two 5 min trials per session
Behavior
Three-chamber social discrimination test, Social defeat
Analysis
Doric Neuroscience Studio, Mosaic, EXTRACT, OASIS
Who to ask
Experimenter
Teruhiro Okuyama
Institution
Institute for Quantitative Biosciences, The University of Tokyo
Notes
Lens implanted immediately after virus injection in the same procedure. Aggressor and nonaggressor positions were swapped between the two trials within each session. Behaviour tracked with EthoVision.