Experiment/sun 2024 mouse ca1 multisensory
From Miniscope
Mouse dorsal CA1 imaging during multisensory stimulation
CA1 imaging across light, auditory, and mixed stimulus blocks alongside linear track running.
Extracted from full textTranscribed by a curator from the published methods section. Values not stated in the paper are marked as not reported rather than inferred.
Animal
Species
Mouse
Strain
C57BL/6J
Sex
Male
Age at surgery
10-12 weeks
Animals
20
Target
Brain region
Coordinates
Injection AP -2.1, ML +2.1, DV -1.7; craniotomy AP -2.1, ML 1.6; lens to DV -1.35 from skull top
Hemisphere
Right
Cell type
CA1 pyramidal neurons
Indicator
Indicator
GCaMP6f
Reports
Calcium
Delivery
AAV injection
Construct
pAAV.Syn.GCaMP6f.WPRE.SV40
Titer
2.2 x 10^13 GC/mL
Injection volume
500 nL
Expression time
5 weeks
Optical access
Method
GRIN lens
Lens
0.23 pitch (Edmund Optics 64-519), through a 2 mm craniotomy
Implant depth
-1.35 mm from skull top
Surgery staging
Separate surgeries
Injection to implant
1 week
Implant to baseplating
5 weeks
Imaging
Condition
Freely behaving
Frame rate
30 fps
Behavior
Linear track, Light stimuli (2 s on / 3-3.5 s off; 300 trials), Auditory stimuli (4/8/16 kHz; 100 trials each), Mixed light-sound stimuli
Analysis
CNMF-E, NoRMCorre, OASIS, MATLAB 2019a, Python 3.7
Who to ask
Experimenter
Chris French
Institution
The University of Melbourne
Notes
Five animals per experiment across four experiments.