Experiment/sun 2022 mouse ca1 transgenic
From Miniscope
Mouse CA1 imaging in a transgenic GCaMP line during conditioned place preference
CA1 imaging in an Ai94 triple-transgenic line — no viral injection — across a drug-context conditioning protocol.
Extracted from full textTranscribed by a curator from the published methods section. Values not stated in the paper are marked as not reported rather than inferred.
Animal
Species
Mouse
Transgenic line
Ai94, Camk2a-tTA, Camk2a-Cre
Sex
Both
Age at surgery
8-12 weeks (19-28 g)
Animals
54
Target
Brain region
Coordinates
AP -2.30, ML +1.75 from bregma; DV -1.53 from skull surface at bregma
Hemisphere
Not reported
Cell type
CA1 pyramidal neurons
Indicator
Indicator
GCaMP6s
Reports
Calcium
Delivery
Transgenic
Optical access
Method
GRIN lens
Lens
1.8 mm diameter x 4.31 mm, 0.25 pitch, 0.55 NA (Edmund Optics)
Implant depth
-1.53 mm
Surgery staging
Not applicable
Implant to baseplating
2 weeks
Imaging
Scope
Condition
Freely behaving
Frame rate
~30 Hz
Sessions
Habituation, baseline, three conditioning pairings, test 1 and test 2 over ~2 weeks
Behavior
Conditioned place preference (methamphetamine; morphine; sucrose)
Analysis
NoRMCorre, CNMF-E, OASIS, MATLAB
Who to ask
Experimenter
Yanjun Sun
Institution
Stanford University School of Medicine
Notes
JAX stock numbers 024115 and 005359. Germline expression removes the injection surgery and the expression-time variable entirely — a useful comparison point for anyone weighing transgenic against viral delivery.