Experiment/schuette 2022 mouse ca1 gabaergic
From Miniscope
Mouse dorsal CA1 imaging comparing GABAergic and glutamatergic populations
The same CA1 preparation run with a CAMK2A promoter and with a VGAT-Cre-dependent construct, to compare stability of the two populations across context change.
Extracted from full textTranscribed by a curator from the published methods section. Values not stated in the paper are marked as not reported rather than inferred.
Animal
Species
Mouse
Transgenic line
VGAT-Cre (JAX 028862)
Sex
Both
Age at surgery
2-6 months
Animals
20
Target
Brain region
Coordinates
AP -2.1 from bregma, ML 1.6 from midline, DV -1.60 from skull
Hemisphere
Not reported
Cell type
CAMK2A-expressing glutamatergic neurons, VGAT-expressing GABAergic neurons
Indicator
Indicator
GCaMP6f
Reports
Calcium
Delivery
AAV + transgenic
Construct
AAV9.CAMK2A.GCaMP6f (Addgene 107790); AAV9.DIO.EF1a.GCaMP6f (Addgene 128315)
Injection volume
500 nL at 80 nL/min
Expression time
~2.5 weeks to imaging (4 days injection to implant, 2 weeks implant to imaging)
Optical access
Method
GRIN lens
Lens
1.8 mm diameter, 0.25 pitch, 0.50 NA (Edmund Optics)
Implant depth
1.35 mm ventral to skull
Surgery staging
Separate surgeries
Injection to implant
4 days
Implant to baseplating
2 weeks
Imaging
Condition
Freely behaving
Frame rate
30 fps
Sessions
3 days linear track, 5 days rotated track, 3 days short-long track
Behavior
Linear track, Rotated track, Short-long track
Analysis
CNMF-E, CellReg, DeepLabCut, MATLAB
Cell yield
2,303 CAMK2A cells; 937 VGAT cells in a linear track session
Who to ask
Experimenter
Avishek Adhikari
Institution
University of California, Los Angeles
Notes
Cohorts: 8 CAMK2A, 12 VGAT. A useful direct comparison of cell yield between a pan-excitatory promoter and a Cre-gated interneuron population in the same preparation — roughly 2.5x more cells from the glutamatergic construct.