Experiment/reis 2021 mouse dpag
From Miniscope
Mouse dorsal periaqueductal gray imaging during threat exposure
dPAG imaging across elevated plus maze and live-rat exposure to compare innate and conditioned threat responses.
Extracted from full textTranscribed by a curator from the published methods section. Values not stated in the paper are marked as not reported rather than inferred.
Animal
Species
Mouse
Strain
C57BL/6J
Sex
Male
Age at surgery
8 weeks
Animals
8
Target
Brain region
Coordinates
AP -4.20, ML -0.85, DV -2.3, at a 15 degree angle
Indicator
Indicator
GCaMP6s
Reports
Calcium
Delivery
AAV injection
Construct
AAV9.Syn.GCaMP6s.WPRE.SV40
Titer
5 x 10^12 viral particles/mL
Injection volume
100 nL
Expression time
~3 weeks to imaging (5 days injection to lens, then 2 weeks to baseplate)
Optical access
Method
GRIN lens
Lens
0.5 mm diameter, ~4 mm long (Inscopix)
Implant depth
-2.0 mm ventral to skull surface
Surgery staging
Separate surgeries
Injection to implant
5 days
Implant to baseplating
2 weeks
Imaging
Condition
Freely behaving
Frame rate
7.5 Hz
Sessions
EPM day 1, habituation day 2, toy rat day 3, live rat day 4
Behavior
Elevated plus maze, Live rat exposure
Analysis
MATLAB, Python, custom
Cell yield
107 +/- 19 cells per mouse (EPM); 857 cells total EPM, 713 rat exposure
Who to ask
Experimenter
Avishek Adhikari
Institution
University of California, Los Angeles
Notes
n = 8 animals for elevated plus maze, 7 for rat exposure. Behavior video captured at 30 fps against 7.5 Hz neural acquisition.