Experiment/petter 2023 mouse dentate gyrus
From Miniscope
Mouse dentate gyrus imaging during operant reinforcement
Cre-dependent dentate granule cell imaging during fixed-ratio operant responding for food reward.
Extracted from full textTranscribed by a curator from the published methods section. Values not stated in the paper are marked as not reported rather than inferred.
Animal
Species
Mouse
Transgenic line
D1-Cre
Sex
Male
Age at surgery
8-10 weeks
Animals
5
Target
Brain region
Coordinates
AP -2.0, ML 1.3, DV -2.2 / -2.1 / -2.0 / -1.9 (four penetrations)
Hemisphere
Bilateral
Cell type
D1 receptor-expressing dentate granule cells
Indicator
Indicator
jGCaMP7f
Reports
Calcium
Delivery
AAV + transgenic
Construct
AAV9-syn-FLEX-jGCaMP7f
Injection volume
50 nL per penetration, four penetrations
Expression time
3 weeks
Optical access
Method
GRIN lens
Lens
1 mm diameter x 4 mm (Inscopix)
Implant depth
1.8 mm DV
Surgery staging
Single surgery
Implant to baseplating
3 weeks
Imaging
Condition
Freely behaving
Sessions
8-10 min sessions
Behavior
Fixed-ratio operant conditioning (FR1/FR3/FR5), Two-lever FR1 delay task, Non-contingent reward
Analysis
CaImAn, CNMF-E, custom MATLAB
Who to ask
Experimenter
Henry H. Yin
Institution
Duke University
Notes
Virus distributed over four dorsoventral penetrations rather than a single bolus.