Experiment/madruga 2025 mouse two photon
From Miniscope
Mouse hippocampal and cortical imaging with an open-source two-photon miniscope
CA1, dentate gyrus, and retrosplenial cortex imaged in freely behaving mice through cranial windows and hippocampal cannulae with a head-mounted two-photon microscope.
Extracted from full textTranscribed by a curator from the published methods section. Values not stated in the paper are marked as not reported rather than inferred.
Animal
Species
Mouse
Sex
Both
Age at surgery
Adult, older than P60
Target
Brain region
Coordinates
Retrosplenial: AP -1.7 and -2.3 from bregma, ML 0.5, DV -0.8 from skull
Hemisphere
Not reported
Indicator
Indicator
GCaMP6f, jGCaMP7f, jGCaMP8f, mCherry
Reports
Calcium, Structural
Delivery
AAV injection
Construct
pGP-AAV-syn-jGCaMP7f-WPRE; pGP-AAV-syn-jGCaMP8f-WPRE; AAV1.Syn.GCaMP6f.WPRE.SV40; AAV1.cFos-tTA; AAV1.TRE.mCherry
Injection volume
1000 nL (CA1); 500 nL (dentate gyrus); 20-120 nL/min (retrosplenial)
Expression time
14 days
Optical access
Method
Cranial window, GRIN lens over cannula
Lens
Cortex: 4 x 4 mm #0 coverslip. Hippocampus: titanium cannula with a 3 mm #0 glass coverslip.
Surgery staging
Separate surgeries
Injection to implant
14 days
Imaging
Scope
Condition
Freely behaving
Frame rate
7.92-8.62 Hz
Sessions
24-60 min recording sessions
Behavior
Open field exploration, Free foraging for sucrose pellets
Analysis
Suite2p, DeepLabCut, ScanImage, MATLAB
Cell yield
CA1: 110 +/- 8 active neurons. Dentate gyrus: ~50 granule cells.
Who to ask
Experimenter
Peyman Golshani
Lab PI
Institution
University of California, Los Angeles
Resources
Notes
Two-photon rather than one-photon, which is why dentate gyrus at over 620 um depth is reachable. Frame rate is correspondingly low (around 8 Hz) compared with the 30 Hz typical of one-photon entries in this registry.