Experiment/lin 2022 mouse cortical vasculature
From Miniscope
Mouse cortical microvasculature imaging after acquired brain injury
Longitudinal imaging of cortical blood vessels rather than neurons, tracking microvascular recovery for two months after contusion or aspiration injury.
Extracted from full textTranscribed by a curator from the published methods section. Values not stated in the paper are marked as not reported rather than inferred.
Animal
Species
Mouse
Transgenic line
Tie2-Cre;Ai9
Sex
Male
Age at surgery
3-4 months
Animals
10
Target
Brain region
Coordinates
AP -1.25 from bregma, ML 1.25 from midline
Hemisphere
Not reported
Cell type
Vascular endothelial cells
Indicator
Indicator
tdTomato, FITC-dextran
Reports
Structural
Delivery
Transgenic
Optical access
Method
GRIN lens
Lens
1.8 mm diameter x 4.31 mm, 0.25 pitch, 0.55 NA
Implant depth
~200-300 um
Surgery staging
Single surgery
Imaging
Condition
Anesthetized
Frame rate
60 Hz
Sessions
Daily imaging for up to 2 months post-injury
Behavior
Cortical contusion injury and aspiration injury models
Analysis
AngioTool v0.6, ImageJ
Who to ask
Experimenter
Andre Obenaus
Institution
University of California, Irvine
Notes
Included as a non-neuronal preparation: the label is a structural endothelial reporter plus an injected vascular dye, not an activity sensor. Lens implanted immediately after injury in the same procedure.