Author Topic: GCaMP transgenic lines  (Read 5039 times)

clay

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GCaMP transgenic lines
« on: November 03, 2016, 02:57:16 PM »
Hey guys- anyone using any GCaMP transgenic lines with the miniscope?
I think consensus is that ROSA lines are too weak, but has anyone tried Allen TIGR/TITL or Thy1:GCaMP lines?
And has anyone seen any RCaMP/jRGECO lines yet that work? Might be good to cut down on autofluorescence...

Walter

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Re: GCaMP transgenic lines
« Reply #1 on: May 23, 2017, 02:19:28 AM »
Hi Clay,

I have been able to measure neuronal activity in CA1 using the Thy1:GCaMP6s (line 4.12, http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0108697) using the finchScope and with a high resolution microscope we designed in our lab. I have not tested it with the UCLA miniscope but I am sure it will work as well.

I think the main problem is that the labeling density is nowhere as high as using AAVs, but there are several advantages to using transgenic animals.

If you are still interested I can test the UCLA miniscope and let you know.