Miniscope Board

General Category => General Discussion => Topic started by: clay on November 03, 2016, 02:57:16 PM

Title: GCaMP transgenic lines
Post by: clay on November 03, 2016, 02:57:16 PM

Hey guys- anyone using any GCaMP transgenic lines with the miniscope?
I think consensus is that ROSA lines are too weak, but has anyone tried Allen TIGR/TITL or Thy1:GCaMP lines?
And has anyone seen any RCaMP/jRGECO lines yet that work? Might be good to cut down on autofluorescence...

Title: Re: GCaMP transgenic lines
Post by: Walter on May 23, 2017, 02:19:28 AM

Hi Clay,

I have been able to measure neuronal activity in CA1 using the Thy1:GCaMP6s (line 4.12, http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0108697) using the finchScope and with a high resolution microscope we designed in our lab. I have not tested it with the UCLA miniscope but I am sure it will work as well.

I think the main problem is that the labeling density is nowhere as high as using AAVs, but there are several advantages to using transgenic animals.

If you are still interested I can test the UCLA miniscope and let you know.