Calibration

[ryancameron]

Hello all, I have been trying to calibrate two of our miniscope systems for a month now and have not had any success. I have used 1.0mm diameter by 9 mm long GRIN lenses as well as .6mm x 7.5mm GRIN lens. I have tried imaging with the calibration slide at all of the focal heights the Miniscope will allow, and still can't get a clear picture of the lines on the slide.

I was wondering if I could get in direct contact with someone who has successfully calibrated the system, so I could take a look at the procedures to try and find where I went wrong.

Thanks!
Ryan Cameron

[Daniel Aharoni]

Hi Ryan,
Just to clarify, are you trying to imaging with only these thin (1mm diameter or less) GRIN lenses or are you imaging in conjunction with a larger (1.8 or 2mm diameter) objective GRIN lens. You need the objective GRIN lens in place to see anything.

[ryancameron]

Okay, that's probably our problem. What specs are the objective GRIN lenses usually? And will that length change depending on the size of our implanted (in the animal) lens?

Thank you!
Ryan Cameron

[Daniel Aharoni]

Hi Ryan,
The relevant GRIN lens information you need can be found here http://miniscope.org/index.php/GRIN_Lens_Information and here http://miniscope.org/index.php/Imaging_With_Thin_GRIN_Lenses.