Topics

1

Optics / Relation of scope slider direction and achromatic FL

« on: September 11, 2017, 09:31:33 PM »

What is the relationship between the direction that you would want to move the miniscope slider in, and the focal length of the achromatic lens?  I.e., if you are consistently wanting to move the slider so far that it falls out (too far up), would you want a larger or smaller focal length of your achromatic lens?

2

Optics / Longer GRIN lens

« on: March 01, 2017, 11:18:14 PM »

Hello,
We are trying to get the imaging working in rats.  One issue is that the 0.25 pitch lenses are too short.  Are there any ~2mm diameter relay lenses?  Are there 0.75 pitch GRIN lenses? 

3

Data Analysis / Background analysis questions

« on: November 09, 2016, 07:46:10 PM »

I'm running the analysis toolbox on Animal G95/Day7/H19_M30_S21.  I have some background questions:

What are the units of ms.time?  It seems that it is taken from timestamp.dat.  What is the units of sysClock?

What is the difference between ms.trace and ms.originalTrace?

Thank you for your help.

4

Surgery and Baseplating / Histology

« on: November 03, 2016, 09:12:14 PM »

To assess efficacy of the virus, one would do histology a month after the injection.  Is there anything that needs to be added to the preparation such as an antibody or tag?  Or does the GCaMP just fluoresce on its own after fixation?

5

Surgery and Baseplating / Rat

« on: September 26, 2016, 04:02:46 PM »

Has there been any success with getting the imaging working in rats?  What virus do you use?  Any changes to the protocol?
Thank you

6

Electronics / PCB questions

« on: August 17, 2016, 05:29:28 PM »

1) Is it possible to get the BOM for the PCB components?

2) Is there any difference between the CMOS PCB v3.0 and v3.2 besides the coax soldering hole arrangement?  I was thinking of soldering the SMA connector directly onto the board as I am using rats and don't mind the extra weight. 

7

Electronics / Coax communication frequency

« on: August 16, 2016, 08:34:42 PM »

What is the frequency of the communication over the coax cable?