Difference between revisions of "Main Page"

From UCLA Miniscope
Jump to: navigation, search
Line 7: Line 7:
  
 
Our miniature fluorescence microscope uses wide-field fluorescence imaging to record neural activity and network structure in awake, freely behavin mice. The functional design of our microscope is similar to a table top wide-field fluorescence microscope with the following key differences:
 
Our miniature fluorescence microscope uses wide-field fluorescence imaging to record neural activity and network structure in awake, freely behavin mice. The functional design of our microscope is similar to a table top wide-field fluorescence microscope with the following key differences:
1. All nonessential componets have been removed
+
*All nonessential componets have been removed
2. The table top objective was replaced with an imaging objective GRadient Index of Refraction (GRIN) lens
+
*The table top objective was replaced with an imaging objective GRadient Index of Refraction (GRIN) lens
3. The miniature fluorescence microscope does not use any infinity optics
+
*The miniature fluorescence microscope does not use any infinity optics
4. The excitation light source is a super bright LED rather than a lamp or laser
+
*The excitation light source is a super bright LED rather than a lamp or laser
5. The optical filters and dichroic mirror are custom cut to minimize their size and weight
+
*The optical filters and dichroic mirror are custom cut to minimize their size and weight
6. A small CMOS imaging sensor is used to record emission light instead of a large CCD, emCCD, or sCMOS camera.
+
*A small CMOS imaging sensor is used to record emission light instead of a large CCD, emCCD, or sCMOS camera.
7. Adjustment of focal plane is done by moving imaging sensor not objective
+
*Adjustment of focal plane is done by moving imaging sensor not objective
  
  

Revision as of 13:33, 16 February 2015

UCLA MINIscope Resource

Welcome to the MINIscope Wiki page. If you do not have an account yet, please request one here.

BaseplateView.JPG

Overview

Our miniature fluorescence microscope uses wide-field fluorescence imaging to record neural activity and network structure in awake, freely behavin mice. The functional design of our microscope is similar to a table top wide-field fluorescence microscope with the following key differences:

  • All nonessential componets have been removed
  • The table top objective was replaced with an imaging objective GRadient Index of Refraction (GRIN) lens
  • The miniature fluorescence microscope does not use any infinity optics
  • The excitation light source is a super bright LED rather than a lamp or laser
  • The optical filters and dichroic mirror are custom cut to minimize their size and weight
  • A small CMOS imaging sensor is used to record emission light instead of a large CCD, emCCD, or sCMOS camera.
  • Adjustment of focal plane is done by moving imaging sensor not objective


Links to MINIscope subsystems

Microscope Components

Software

Surgery

Processing

Part Lists