Initial Testing of Assembled Miniscopes

This page is a work in progress. Once your Miniscope system is up and running, it is important to be able to test, and debug, all aspects of the system before moving to imaging in vivo. The sections below will discuss the procedures we use to validate Miniscopes we build before using them in experiments. As you become more comfortable with using Miniscopes some of the sections below can be skipped.

Imaging Calibration Slides

Image of a calibration slide with 9.8μm line spacing (superimposed red boxes are 10px x 10px)