Initial Testing of Assembled Miniscopes

This page is a work in progress. Once your Miniscope system is up and running, it is important to be able to test, and debug, all aspects of the system before moving to imaging in vivo. The sections below will discuss the procedures we use to validate Miniscopes we build before using them in experiments. As you become more comfortable with using Miniscopes some of the sections below can be skipped.

Testing the Coaxial Cable Connection

In our experience, the connection of the coax cable, either to the CMOS Imaging Sensor PCB or SMA connector, is by far the most common source of power or data transmission issues

Imaging Calibration Slides

Image of a calibration slide with 9.8μm line spacing (superimposed red boxes are 10px x 10px)