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From UCLA Miniscope
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Our miniature fluorescence microscope uses wide-field fluorescence imaging to record neural activity and network structure in awake, freely behavin mice. The functional design of our microscope is similar to a table top wide-field fluorescence microscope with the following key differences: | Our miniature fluorescence microscope uses wide-field fluorescence imaging to record neural activity and network structure in awake, freely behavin mice. The functional design of our microscope is similar to a table top wide-field fluorescence microscope with the following key differences: | ||
| − | *All nonessential | + | * All nonessential components are removed |
| − | *The table top objective | + | * The table top objective is replaced with an imaging objective GRadient Index of Refraction (GRIN) lens |
| − | + | * The excitation light source is a super bright LED rather than a lamp or laser | |
| − | *The excitation light source is a super bright LED rather than a lamp or laser | + | * The optical filters and dichroic mirror are custom cut to minimize their size and weight |
| − | *The optical filters and dichroic mirror are custom cut to minimize their size and weight | + | * A small CMOS imaging sensor is used to record emission light instead of a large CCD, emCCD, or sCMOS camera |
| − | *A small CMOS imaging sensor is used to record emission light instead of a large CCD, emCCD, or sCMOS camera | + | * Adjustment of focal plane is done by moving imaging sensor not objective |
| − | *Adjustment of focal plane is done by moving imaging sensor not objective | ||
Revision as of 12:35, 16 February 2015
UCLA MINIscope Resource
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Overview
Our miniature fluorescence microscope uses wide-field fluorescence imaging to record neural activity and network structure in awake, freely behavin mice. The functional design of our microscope is similar to a table top wide-field fluorescence microscope with the following key differences:
- All nonessential components are removed
- The table top objective is replaced with an imaging objective GRadient Index of Refraction (GRIN) lens
- The excitation light source is a super bright LED rather than a lamp or laser
- The optical filters and dichroic mirror are custom cut to minimize their size and weight
- A small CMOS imaging sensor is used to record emission light instead of a large CCD, emCCD, or sCMOS camera
- Adjustment of focal plane is done by moving imaging sensor not objective
