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== Overview ==
 
== Overview ==
  
Our miniature fluorescence microscope uses wide-field fluorescence imaging to record neural activity and network structure in awake, freely behavin mice. The functional design of our microscope is similar to a table top wide-field fluorescence microscope with the following key differences:
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Our miniature fluorescence microscope uses wide-field fluorescence imaging to record neural activity and network structure in awake, freely behavin mice.  
* All nonessential components are removed
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[[Overview of system]]
* The table top objective is replaced with an imaging objective GRadient Index of Refraction (GRIN) lens
 
* The excitation light source is a super bright LED rather than a lamp or laser
 
* The optical filters and dichroic mirror are custom cut to minimize their size and weight
 
* A small CMOS imaging sensor is used to record emission light instead of a large CCD, emCCD, or sCMOS camera
 
* Adjustment of focal plane is done by moving imaging sensor not objective
 
 
 
  
 
== Links to MINIscope subsystems ==
 
== Links to MINIscope subsystems ==

Revision as of 12:37, 16 February 2015

UCLA MINIscope Resource

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BaseplateView.JPG

Overview

Our miniature fluorescence microscope uses wide-field fluorescence imaging to record neural activity and network structure in awake, freely behavin mice. Overview of system

Links to MINIscope subsystems

Microscope Components

Software

Surgery

Processing

Part Lists