Difference between revisions of "Cortex buffer"
From UCLA Miniscope
(Created page with "Cortex Buffer Recipe: (buffered artificial cerebrospinal fluid) In 1000 ml milipore water: NaCl = 7.888g KCl = .372g HEPES = 1.192g CaCl2 = .264g MgCl2 = .204g pH to 7.4 a...") |
|||
| Line 2: | Line 2: | ||
In 1000 ml milipore water: | In 1000 ml milipore water: | ||
| + | |||
NaCl = 7.888g | NaCl = 7.888g | ||
| + | |||
KCl = .372g | KCl = .372g | ||
| + | |||
HEPES = 1.192g | HEPES = 1.192g | ||
| + | |||
CaCl2 = .264g | CaCl2 = .264g | ||
| + | |||
MgCl2 = .204g | MgCl2 = .204g | ||
pH to 7.4 and filter. | pH to 7.4 and filter. | ||
Latest revision as of 14:23, 18 January 2016
Cortex Buffer Recipe: (buffered artificial cerebrospinal fluid)
In 1000 ml milipore water:
NaCl = 7.888g
KCl = .372g
HEPES = 1.192g
CaCl2 = .264g
MgCl2 = .204g
pH to 7.4 and filter.
