Hi JCima,
Great questions.
All 0.25pitch GRIN lenses have an infinity working distance. What this means is a point source of light sitting on the bottom surface of the lens will produce parallel light rays (what is sometimes referred to as infinity optics or infinity space) coming out of the other end of the lens. This is similar to how a standard objective lens of a table top microscope works. The achromatic lens, or tube lens, then focuses this parallel light into an image on the CMOS imaging sensor. The combination of the GRIN lens and tube lens defines the functional working distance of the scope.
As the point source of light moves away from the bottom surface of the GRIN lens, the exiting light from the lens starts to deviate from being parallel. This effectively shifts the location of the image produced by the achromatic lens. Or thinking about this in reverse, if you shift the z-height of the CMOS imaging sensor, you effectively shift the plane in focus below the GRIN lens. Roughly, an overall shift of 6mm of the CMOS sensor will shift the focal plane in the brain by about 200um.
I hope this answers your questions.