Recent Posts

11

Electronics / Re: Coax communication frequency

« Last post by ONECore on January 04, 2019, 10:34:22 PM »

Instructions for an inexpensive passive commutator tested with the Miniscope can be found: https://optogeneticsandneuralengineeringcore.gitlab.io/ONECoreSite/projects/SlickPassiveElectricalCommutator/

12

Electronics / Re: How to attach commutator?

« Last post by ONECore on January 04, 2019, 10:33:10 PM »

Instructions for an inexpensive passive commutator tested with the Miniscope can be found: https://optogeneticsandneuralengineeringcore.gitlab.io/ONECoreSite/projects/SlickPassiveElectricalCommutator/

13

Behavior / Re: Swivel/tether for hands-off recording

« Last post by ONECore on January 04, 2019, 10:32:10 PM »

Instructions for an inexpensive passive commutator tested with the Miniscope can be found: https://optogeneticsandneuralengineeringcore.gitlab.io/ONECoreSite/projects/SlickPassiveElectricalCommutator/

14

General Discussion / Happy New Year 2019

« Last post by hsiao on January 01, 2019, 04:26:03 AM »

Hello everyone,

Wish everybody has remarkable success in your experiments.

I'll keep working on Ca imaging this year, too.

I am curious about the difficulty of seeing Ca imaging in mice.
Does anyone like to talk about your percentage of successful seeing signal?

I am especially interested in the dorsal, ventral hippocampus and the amygdala.

I hope someone can give me some directions.


Thanks!

15

Electronics / Re: Excitation LED got too weak after I replaced a new coax cable

« Last post by Daniel Aharoni on November 16, 2018, 04:03:32 AM »

Hi qdong8,
Can you tell me the specs of the new cable or post a link to it? If it is extremely thin then there is a chance it could decrease the total amount of current the LED can draw.

16

Electronics / Excitation LED got too weak after I replaced a new coax cable

« Last post by qdong8 on November 14, 2018, 02:50:51 AM »

Recently I replaced the original coax cable (miniscope bought from Lab Maker) with a long cable (2 meters). After that, the LED got too dim. Have you ever met such issues? Thanks!

17

Behavior / Re: Sync Behavior - More TTLs sent than frames recorded

« Last post by hahnj on November 05, 2018, 02:24:17 PM »

Dear Daniel,

Thanks, I will have a look.

18

Behavior / Re: Sync Behavior - More TTLs sent than frames recorded

« Last post by Daniel Aharoni on October 30, 2018, 09:42:27 PM »

Hi hahnj,
This is due to the computer's USB hardware not being able to keep up with the data being sent out of the Miniscope DAQ. There are a couple ways to correct for this and luckily David Tank's group just published a paper with a detailed supplement on how to deal with this. Here is a link to that info: https://www.cell.com/neuron/fulltext/S0896-6273(18)30852-3. Section B-2 in the supplemental materials.

Hope this helps.

19

Behavior / Sync Behavior - More TTLs sent than frames recorded

« Last post by hahnj on October 29, 2018, 02:33:30 PM »

I realized that when I record the TTLs that the miniscope sends for each recorded frame I always end up with more pulses/timestamps than frames recorded. The difference ranges from very few up to more than 100.

I couldn't find any correlation between the difference and recording length.
It is always more timestamps than frames.
The timestamp.dat file corresponds to the number of frames recorded.
The time difference between all TTL-timestamps recorded is the same (so I cannot exclude some timestamps just by looking at the difference).
=> It looks like the Miniscope sends the TTLs before starting the recording or is still sending TTLs after the Recording stopped (Or both).

Any ideas what could cause this or possible solutions?

 

20

Optics / Re: Non Uniform illumination or vignetting in miniscope?

« Last post by kyrollosyanny on October 22, 2018, 01:00:11 AM »

Thank you for the reply